Global Quantitative Protein Analysis [S-0010]
Two Dimensional – Difference In Gel Electrophoresis (2D-DIGE) is a gel-based approach for comparative proteomics using fluorescent tags. Distinct fluorescent tags e.g. Cy 3, 5 and 2 are used to label samples and a universal internal standard prior to 1st/2nd dimension electrophoresis. An automated software program is used to detect, quantify and annotate differentially expressed proteins. 2D-DIGE offers all the advantages of 2D-PAGE and overcomes the inherent disadvantage of variation and reproducibility problem in a 2D-PAGE.
Our quantitative and comparative proteomics using 2D DIGE (two-dimensional difference gel electrophoresis) services include:
a) experimental design,
b) sample preparation to isolate total proteins using ToPI-DIGE protein isolation Kit,
c) fractionation, quantitation and qualification of proteins if applicable,
d) 2D-DIGE analysis, scanning of gels using a DIGE-enabled digital scanner and
e) biological variation analysis of gel images to identify differentially expressed proteins with statistically significant difference.
Cost is per biological or clinical specimen.
Contact Us for more information on this service.
2D-DIGE SERVICES
- Global Quantitative Protein Analysis by 2D-DIGE [S-0010]
- Candiate spot picking using an automated spot picking robot [S-0010.1]
- Serum/plasma depletion of 14 abundant proteins using IgY [S-0045.1]
- 2D Gel Western Blotting on PVDF or Nitrocellulose Membranes
- Phosphoprotein expression using 2D Gels
- Glycoprotein expression using 2D Gels
*Example
Compartive proteomics of diploid and haploid isolates of Rhizoctonia Solani Mycelium are being analyzed through 2D-DIGE services.
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