Two Dimensional Difference in-Gel Electrophosis

ITSI-Biosciences utilizes validated sample preparation kits/protocols, robotic spot pickers/digesters, difference-in-gel analysis software and advanced mass spectrometer to offer an integrated solution from sample preparation to differential protein annotation. Data obtained after 2D-DIGE experiment may include information on identity of the protein, pI, molecular weight and post translational modifications. 

ITSI-Biosciences offer the 2D-DIGE services to both industrial and academic clients. Our decade of experience with 2D-DIGE process enables us to offer reproducible picture perfect gels at a competitive price and guaranteed faster turnaround time. We have also developed a variety of complementary kits to streamline and standardize the upstream sample preparation steps prior to 2D-DIGE, thereby reducing the overall time and cost of performing 2D-DIGE experiments.

2D-DIGE SERVICES

• Global Quantitative Protein Analysis by 2D-DIGE [S-0010]
• Candiate spot picking using an automated spot picking robot [S-0010.1]
• Serum/plasma depletion of 14 abundant proteins using IgY [S-0045.1]
• 2D Gel Western Blotting on PVDF or Nitrocellulose Membranes
• Phosphoprotein expression using 2D Gels
• Glycoprotein expression using 2D Gels

other services