Featured Protein Analysis by 2D-DIGE
Two Dimensional – Difference In Gel Electrophoresis (2D-DIGE) is a gel-based approach for comparative proteomics using fluorescent tags. Distinct fluorescent tags e.g. Cy 3, 5 and 2 are used to label samples and a universal internal standard prior to 1st/2nd dimension electrophoresis. An automated software program is used to detect, quantify and annotate differentially expressed proteins. 2D-DIGE offers all the advantages of 2D-PAGE and overcomes the inherent disadvantage of variation and reproducibility problem in a 2D-PAGE.
The scientists at ITSI-Biosciences had very early access to the 2D-DIGE technology in 2001; worked closely with Amersham Biosciences (now GE Healthcare) in the early days of commercialization in both promoting/validating the technology, and pioneering some key modifications in the workflow process e.g. recommending the switch to the Typhoon Digital Imager as the scanner for capturing 2D-DIGE images rather than the originally supplied scanner which bleached 2D-DIGE gels.
For more details about this service please check here.
Featured Mass Spectrometry
Liquid chromatography coupled to mass spectrometry (LC MS/MS) has become the backbone for understanding biochemical pathways to discovering new biomarkers for debilitating diseases. ITSI Biosciences has been utilizing advanced LC-MS/MS technologies to provide unsurpassed protein identification and characterization services for more than 6 years. The services are offered to both industrial and university clients at a competitive price and faster turnaround time.
We utilize state of the art techniques and constantly update our skills and equipment to accommodate the constantly evolving proteomics field. Some of the areas of proteomics which we specialize in are listed below. This list is just for reference and is not exhaustive. Please don't hesitate to call us for techniques not listed here.
- Protein Identification & Characterization by LC/MS/MS
- In-gel digestion of protein spot with protein sequencing by mass spectrometry
- In-solution digestion of protein sample with sequencing by mass spectrometry
- Post Translational Modification Mapping by Mass Spectrometry
- Phosphoprotein / Peptide Analysis
- SCX Mudpit Analysis
- iTRAQ Mass Spectrometry Service
- Analysis of Serum/Plasma after depletion of 14 abundant proteins using lgY column
- Analysis of Serum/Plasma after depletion of Albumin and lgG using the ASKc Column
- Mass spectrometry Analysis of Urine samples
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1) In-gel-Digestion,
2)In solution/GelFree Digest,
3) Phosphopeptide Identification,
4)Biomarker Analysis (Plasma/Serum Depletion), 5)Quantification.
Services List
2d-dige services
- Global Quantitative Protein Analysis by 2D-DIGE [S-0010]
- Whole Proteome Screening [WPS-010]
- Candidate spot picking using an automated spot picking robot [S-0010.1]
- Serum/plasma depletion of 14 abundant proteins using IgY [S-0035.1]
- 2D Gel Western Blotting on PVDF or Nitrocellulose Membranes
- Phosphoprotein expression using 2D Gels
- Glycoprotein expression using 2D Gels
Mass specTROMETRY services
- Protein Identification & Characterization by LC/MS/MS [S-0030]
- In-gel digestion of protein spot with protein sequencing by mass spectrometry [S-0010.2]
- In-solution digestion of protein sample with sequencing by mass spectrometry [S-0031]
- Post Translational Modification Mapping by Mass Spectrometry [S-0032]
- Phosphoprotein / Peptide Analysis [S-0033]
- SCX Mudpit Analysis [S-0034]
- iTRAQ Mass Spectrometry Service [S-0037.1]
- Analysis of Serum/Plasma after depletion of 14 abundant proteins using lgY column[S-0037.2]
- Analysis of Serum/Plasma after depletion of Albumin and lgG using the ASKc Column[S-0037.3]
- Mass Spectrometry Analysis of Urine Samples [S-0038.1]
SAMPLE PREP services
- Serum/plasma depletion of 14 abundant proteins using IgY [S-0035.1]
- ITRAQ Labeling - 2-Plex [S-0036.1]
- Isolating protein from Urine using inhouse Kit (ToPI-U kit) [S-0038]
- Depletion of Albumin and lgG using the ASKc Column Kit
- Depletion of Albumin and lgG using the ASKs Kit
Multi-analyte analysis-XMAP
Important Info
Sample Submission FormClick here to download the project summary form.
Frequently Asked Questions FAQ's about our different services.
Recent ProjectsWant to view a list of our past projects? Click here!
