Albumin Segregation Kit-Solvent [k-0013]

Human serum albumin (66 KDa) typically accounts for more than 65% of the total protein present in plasma and serum samples. High albumin concentration obscures the detection of low abundance plasma and serum proteins in most experiments. Albumin also distorts lanes during electrophoresis if a large amount of total protein is loaded to permit the detection of low abundant proteins. Albumin can also mask other proteins that migrate around the 50-70 kDa thereby preventing their detection.

ITSIPrep ASKs (K-0013) is a validated and cost effective organic solvent-based protocol for selective depletion of albumin (Alb) from serum and plasma samples and analysis of the albumin-enriched fraction (Alb+) and albumin depleted (Alb-) fractions. More than 85% of albumin is depleted from whole plasma using the ITSIPrep ASKs Kit and Protocol (see Figure). This protocol has been tested and successfully used to improve the chances of revealing lesser abundant proteins in the Alb- fraction or albumin-associated proteins in the Alb+ fraction. Treated samples can be analyzed by electrophoresis (1D-PAGE, 2D-PAGE, 2D-DIGE), mass spectrometry and western analysis.

  • 1D-PAGE gel image of Human Plasma SM is size marker and WP is whole plasma. A, B and C are triplicate experiments.The lane marked 1 represent albumin depleted fractions (A-) and lanes marked 2 represent albumin enriched fraction (A+). The albumin band is indicated with red arrow. More than 85% of albumin is depleted from whole plasma using the ITSIPrep ASKs Kit and Protocol without eliminating other proteins.

Each Kit contains optimized buffer systems and consumables including:

  • ASKs Buffer 1
  • ASKs Buffer 2
  • ASKs Buffer 3
  • ProPreCip Precipitation Booster
  • Microgrinder pestles and tubes

 

K-0013-50 (50 Assays) - $119.38 Visit the Online store to purchase product.Order

Useful Links

  • Material Safety Data Sheet Click here to Download
  • Standard ProceduresClick here to Download