Protein ID & Characterization by LC/MS/MS

nano-LC/MS/MS

ITSI Biosciences offers protein sequencing services for protein identification, protein quantitation and for identifying post translational modifications (PTM).  ITSI Biosciences utilizes nano LC/MS/MS technology for all the protein identifications. Hence we can tailor our LC to analyze from a simple gel plug to a complex sample like cell lysate or plasma. To increase coverage of protein identification on a complex sample like serum, plasma, whole cell lysate and tissue homogenate we utilize Multi-Dimensional Protein Identification Technology (MudPIT) technology to reduce sample complexity.

Protein quantitation is another big area offered along with protein identifications. We can do both label free approach - spectral counting or ion intensity measurements or labeled approach using Isobaric tags for relative and absolute quantitation (iTRAQ). We have successfully finished multiple projects this year utilizing either label free approach or iTRAQ based biomarker identifications and we provide results either as relative abundance (iTRAQ ratios) or absolute quantities (pmol, pg, ug or fg) for label free method .

Comprehensive identification of post translational modifications (PTM) is the third most sort after service at ITSI Biosciences. We offer simple PTM analysis utilizing a multistage activation approach. For extensive PTM analysis we use either immobilized metal affinity chromatography (IMAC) or Titanium dioxide (TiO2) for phosphopeptide enrichment. For very complex  samples, ITSI Biosciences starts with  Strong Cation Exchange (SCX) fractionation followed by IMAC or TiO2 phosphoenrichment.  For lysine rich proteins or proteins without trypsin cleavable sites, we utilize a variety of nontraditional enzymes like LysC, ArgC, AspN and GluC for good coverage and PTM results. To ensure that proteins are sufficiently digested into peptides prior to LC/MS/MS, ITSI-Biosciences utilizes the proprietary ProDM kit (http://www.itsibio.com/protein_digestion.html) to accurately calculate the percentage of protein digestion into peptides.    

Our team can perform global proteomics, protein expression profiling, comparative proteomics and targeted proteomics for biomarker discovery and monitoring. Most biological and clinical samples can be analyzed directly or after electrophoresis (e.g. 2D-DIGE, SDS-PAGE) and candidate proteins can be identified  by nano LC/MS/MS after in-gel or in-solution digestion.

Our Protein Identification services by Mass Spectrometry include:

For a 90 Minute Run (Cat# S-0030)

    a) Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • b) Database search
  • c) Identified full protein annotation.

Cost is per Sample.

For a 180 - 240 Minute Run (Cat# S-0030.1)

    a) Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • b) Database search
  • c) Identified full protein annotation.

Cost is per Sample.

Contact Us for more information on this service.

LC/MS/MS SERVICES

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