Protein Identification In-gel Digestion [S-0010.2]

In-gel Digestion:Gel Electrophoresis provides a reproducible, affordable and reliable way to fractionate proteins based on molecular weight (SDS-PAGE), or molecular weight and pI (2DE, 2D-DIGE) to reduce protein sample complexity.  Our in-gel digestion workflow includes spot picking, digestion and mass spec analysis from 1D and 2D gels.

Our services includes:

a) Performance of preparative gels
b) Staining of gels with fluorescent dye,
c) Scanning of gels with Typhoon Digital Imager,
d) Matching of preparative gels to analytical gels to identify spots of interest,
e) Picking and digestion of protein spots,
f) In-gel digestion of protein spots,
g) Tandem mass spectrometry analysis using nano-LC/MS/MS,
h) Database search
i) Full protein annotation including GO annotations

Cost is per spot.

Apart from this service we offer a unique kit (Protein Digestion Monitoring kit (ProDM)) to check for the completion of trypsin digestion without mass spectrometry. This is a valuable kit for clients mailing precious samples who don't have an in-house and inexpensive method to check for the completion of trypsin digestion.

Contact Us for more information on this service.

LC/MS/MS SERVICES

• Protein Identification & Characterization by LC/MS/MS [S-0030]
• In-gel digestion of protein spot with protein sequencing by mass spectrometry [S-0010.2]
• In-solution digestion of protein sample with sequencing by mass spectrometry [S-0031]
• Post Translational Modification Mapping by Mass Spectrometry [S-0032]
• Phosphoprotein / Peptide Analysis [S-0033]
• SCX Mudpit Analysis [S-0034]
• iTRAQ Mass Spectrometry Service [S-0037.1]
• Analysis of Serum/Plasma after depletion of 14 abundant proteins using lgY column[S-0037.2]
• Analysis of Serum/Plasma after depletion of Albumin and lgG using the ASKc Column[S-0037.2]
• Mass Spectrometry Analysis of Urine Samples [S-0038.1]

other services