Protein Identification In-solution Digestion [S-0031]

In-solution / gelfree Digest
We offer in-solution digestion for un-fractionated or fractionated (i.e. HPLC or affinity purification) protein samples.  We offer services using traditional trypsin and other proteases including AspN, Glu-C, and ArgC. This approach eliminates time consuming gel electrophoresis and also reduces the loss associated with protein recovery during in-gel digestion. We have expertise to optimize nano-LCMS for working with tryptic peptides and also with non-tryptic peptides including those naturally occurring – (e.g. from cerebrospinal fluid).

Our peptide sequencing and protein identification by tandem mass spectrometry services includes:

For a 90 Minute Run (Cat# S-0031.2)

a) In-solution digestion including reduction ad alkylation
b) Tandem mass spectrometry analysis using nano-LC/MS/MS,
c) Database search,
d) Full protein annotation including GO annotation.
Cost is per sample.

For a 180 - 240 Minute Run (Cat# S-0031.3)

a) In-solution digestion including reduction ad alkylation
b) Tandem mass spectrometry analysis using nano-LC/MS/MS,
c) Database search,
d) Full protein annotation including GO annotations.
Cost is per sample.

Contact Us for more information on this service.

LC/MS/MS SERVICES

• Protein Identification & Characterization by LC/MS/MS [S-0030]
• In-gel digestion of protein spot with protein sequencing by mass spectrometry [S-0010.2]
• In-solution digestion of protein sample with sequencing by mass spectrometry [S-0031]
• Post Translational Modification Mapping by Mass Spectrometry [S-0032]
• Phosphoprotein / Peptide Analysis [S-0033]
• SCX Mudpit Analysis [S-0034]
• iTRAQ Mass Spectrometry Service [S-0037.1]
• Analysis of Serum/Plasma after depletion of 14 abundant proteins using lgY column[S-0037.2]
• Analysis of Serum/Plasma after depletion of Albumin and lgG using the ASKc Column[S-0037.3]
• Mass Spectrometry Analysis of Urine Samples [S-0038.1]

other services