Most biological samples contain a complex mixture of proteins in different regions, e.g. membrane, nucleus and cytoplasm. Therefore, to identify the most complete array of proteins in each region, it is necessary to reduce the complexity of the total protein lysate by using a reliable and reproducible method that will enable the enrichment of proteins that are present in specific locations. Reduction in sample complexity is also particularly important when the analysis of proteins that occur at low concentrations in the membrane, cytosol and nuclear regions of the cell is desired. An ideal method should be reproducible, simple, convenient and cost effective.

ITSIPrep ProFEK (K-0015) Protein Fraction Enrichment Kit (ProFEK) provides a validated, fast, and cost-effective enrichment kit system for isolation and concentration of proteins predominant in the membrane, cytosol, and nuclear regions of mammalian cells and tissues without ultra centrifugation. The optimized reagents and procedure provided with the kit allow for reproducible results and identification of unique region associated proteins. The extracted membrane, nuclear, and/or cytoplasmic protein fractions are suitable for SDS-PAGE (Fig 1), 2D-PAGE, 2D-DIGE (Fig 2), and western blotting.