Cell Proliferation Kit

Recently, colormetric assays has become available for analyzing the number of viable cells by the cleavage of tetrazolium salts added the culture medium. This technique requires neither washing nor harvesting cells and the complete assay from onset of the microculture to data analysis by ELISA reader is performed in the same microtiter plate.

ITSI Cell Proliferation Kit provides very convenient and most sensitive means for quantifying cell proliferation, viability or cytotoxicity. The assay is based on the cleavage of the tetrazolium salt to Water-soluble formazan by succinate-tetrazolium reductase system which belongs the respiratory chain of the mithochondria and active only in the viable cells. Therefore, the amount of the formazan dye is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 460 nm.

  • Application.

    ITSI Cell Proliferation Kit can be used for measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.

  • It is faster, stable, and more sensitive than other tetrazolium salts such as MTT, XTT or MTS.
    ITSI Cell Proliferation Kit is provided as a ready-to-use solution, no premixing of components is required.

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Benefits of using ITSI Cell Proliferation Kit:


  • - Convenient: Ready-to-use format, no premixing of components is required.
    - Safe: No organic solvents or isotopes required.
    - Easy: No harvesting, no washing and no solubilization steps.
    - Sensitive: More sensitive than MTT, XTT orMTS
    - Accurate: The assay correlates well with the [3H]-thymidine incorporation assay.
    - Flexibility: Plate can be read and returned several times to the incubator for further color development.

 

Features:

  • Ready to use, one bottle solution
    Shipping and storage condition: 4℃ (Gel pack)
    Unit: 5ml/vial (for 5 x 96 well microplates)

K-0027-300 (300 Assays) - $460.00 Visit the Online store to purchase product.Order

 

 

 

Useful Links

  • Material Safety Data Sheet Click here to Download
  • Standard ProceduresClick here to Download