RNA Profiling and Quantitation

ITSIBIO utilizes the Luminex xMAP technology and the Panomics QuantiGene™ plex and branched DNA (b-DNA) reagent System to provide RNA profiling services. Multiple RNA targets can be monitored simultaneously in the same samples.

Gene expression profiling is performed with the sensitive and reproducible QuantiGene SinglePlex and MultiPlex technology and reagent systems which has higher sensitivity and does not require RNA isolation, target amplification by PCR and reverse-transcription. This technology can be applied for research in the following areas: cancer, cardiac disease, ccellular signaling, cytokines, chemokines, growth factors, endocrine, matrix metalloproteinases, metabolic disease, neurobiology, transcription factors and nuclear Receptors.

Most biological and clinical samples can be analyzed, including, tissue homogenate, cell lysate, whole blood, purified RNA, serum, plasma, and formalin-fixed paraffin embedded samples.

Partial list of assays available: 1L6R, ACTB, BAD, BAK1 (BAK), BCL2, BCL2L1 (BCL-XL), CDKN1A (CDKN1), CFLAR (CFLIP), CSF2, GAPD, IFN-gamma, IL-1 beta, IL-10, IL-2, IL-6, IL-8, NFKB2, NFKBIA (NFKIA), NKFB1, PPIB, Ptk2B (RAFTK), RELA, RELB, TNF, TNFAIP3 (A20), TNFRSF6 (FAS), TNFSF6 (FASL), VEGF,

• Multiplexing - Up to 100 bioassays per well; including controls.
• Parallel analysis - simultaneous quantitation of multiple proteins in the same sample.
• Sample amount - less than 50ul for up to 100 data points.
• Reproducibility - provides consistent results and allow for easy quality control.
• Accuracy - Quantitative results over a 3 to 4 log dynamic range, with strong concordance to ELISA, mass spectrometry, micro array and real time PCR.