Serum/Plasma depletion using lgY [S-0035.1]

We have both in-house validated albumin depletion kit (ASKc; Cat #K-0012-10 and
ASKs; Cat #K-0013-50) and a 14 proteins depletion kit for depletion of abundant proteins from serum/plasma prior to analysis.

Removing 14 most abundant proteins reduces plasma protein concentration by 90 %. Without depletion it is impossible to identify biomarkers which are usually in the low copy numbers compared to albumin or IgGs. Depleting them increases our ability to identify low abundant proteins and hence candidate biomarkers. 

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RELATED SERVICES

• 2d-dige services

  • Global Quantitative Protein Analysis by 2D-DIGE [S-0010]
  • Candiate spot picking using an automated spot picking robot [S-0010.1]
  • 2D Gel Western Blotting on PVDF or Nitrocellulose Membranes
  • Phosphoprotein expression using 2D Gels
  • Glycoprotein expression using 2D Gels

• SAMPLE PREP SERVICES

  • Isolating Protein from Urine using the ToPI-U Kit [S-0038]
  • ITRAQ Labeling [S-0036]
  • Depletion of albumin and lgG using the ASKc column Kit
  • Depletion of albumin and lgG using the ASKs Kit