GenPlates AutomationGenPlates® are an automation friendly, high density FTA paper-based format for biobanking of blood and other biological fluids. Each GenPlate is in a 384-microplate format with a 6-mm disc of FTA paper in each well. FTA paper is, for decades now, the de-facto standard for protecting DNA that is stored on paper as dried blood spots.

GenPlates is used for storage and protection of DNA in dried blood spots since 2006, and the technology is protected by US and International Patents. GenPlates are available in several formats to best fit the storage needs of your samples. GenPlates are currently used in the USA, Canada, Africa and Asia for storage of human and non-human biological specimens as well as for transportation of critical biospecimens from laboratory to laboratory or across state and national boundaries. The transport of biological specimens in the dry-state and at room temperature eliminates the drudgery and cost of cold chain management with wet-ice and dry-ice, thereby reducing the overall cost of biosample acquisition, transport and storage.

 

GenPlate Technology

 

GenPlates are a high density, proprietary format for storing and transporting biosamples at ambient temperatures, in the dry state, embedded in 6 mm discs of FTA paper.

FTA paper is a macroporous cellulose matrix that is chemically treated to inactivate bacteria and virus. Upon application of 10µl of blood or other biosample directly to the 6mm disc of FTA paper, cells lyse and release DNA. The DNA then becomes entwined in the fibrous network of the cellulose matrix. The entwined biosample is dried completely at room temperature and the dried sample is sealed with an adhesive cover and is ready for storage or transport at ambient temperature.  More than 18 years of data and analysis exist for DNA storage on FTA paper and the chemistry for recovering DNA from FTA paper has been thoroughly validated. DNA stored on and recovered from FTA paper has been reliably used in demanding downstream experiments such as Illumina and Affymetrix microarrays, HLA typing, WGA, PCR, STR and genotyping and NGS.

 

GenPlate FormatsGenPlates

 

The most popular GenPlate format is the 24 region GenPlate that stores blood spots from 24 subjects and each subject has 4 replicates stored on the GenPlate. All current sequencing technologies can now do deep whole genome sequencing on the DNA extracted from only 6 mm disc. All the extra DNA is often put onto GenTegra-DNA to preserve it for later use as needed. The four replicates ensure that another sample is always available when needed.

Dried blood spots on FTA paper require storage in a humidity-controlled environment to ensure that the samples are properly protected from future contamination. GenTegra also supplies cabinets and archives that provide active humidity control for the storage of GenPlates. Tracking of plate and sample location are easy. Each GenPlate has a unique barcode on it and the Personal Archive offers a storage cabinet that provides a unique 2D barcode for each and every storage location within the archive.

Specifications:

  • GenPlates are 384-well half height (ANSI/SLAS 1-2004 standard) microplates
  • Each well of sample region contains a 6mm disc of FTA paper
  • Each disc in the well will hold 10µl of blood, purified genomic DNA (gDNA), purified plasmid DNA (pDNA), crude bacterial suspension (clones), or glycerol bacterial stock
  • Using GenSolve each disc yields approximately 100ng of DNA
  • Each plate is foil sealed top and bottom
  • GenPlates are formatted into five different region patterns
  • Each plate contains a 14-digit barcode which specifies plate type, region configuration, and unique plate number
GenPlate Regions

 

Catalog Numbers: Below are the catalog numbers for the different formats.

Catalog #NameDescriptionRegionsElementsUnitNo Per Pack
GVNP-20 GenPlate 01R384EGenPlate Single Region (384 Element)1384EA20
GVN3P-20 GenPlate 03R96EGenPlate 3 Region (96 Element)396EA20
GVN6P-20 GenPlate 06R40EGenPlate 6 Region (40 Element)640EA20
GVN12P-20 GenPlate 12R16EGenPlate 12 Region (16 Element)1216EA20
GVN24P-20 GenPlate 24R4EGenPlate 24 Region (4 Element)244EA20
GVN24P6-20GenPlate 24R6EGenPlate 24 Region (6 Element) 246EA20
GVSL1-100 GenPlate Replacement SealsGenPlate Replacement Seals 11EA100
GVSL1-20 GenPlate Replacement SealsGenPlate Replacement Seals11EA20
Pricing: Please send an email to itsi@itsibio.com to request a quote