Posted on

types of protein testing


LC-MS/MSLiquid Chromatography-Mass Spectrometry (LC-MS/MS) is an analytical process widely used in proteomics because it combines the physical separation capabilities of liquid chromatography with the mass determination ability of mass spectrometry in one integrated workflow.  LC-MS/MS enables the researcher to simultaneously identify proteins and post translational modifications, as well as perform protein quantitation using a label or label free techniques. LC-MS/MS can be used to analyze simple and complex biological samples.


2D-DIGETwo Dimensional – Difference in Gel Electrophoresis (2D-DIGE) is the most accurate, reproducible, and flexible gel-based approach for comparative and quantitative proteomics using distinctive fluorescent tags. With 2D-DIGE, 2 – 3 samples can be analyzed on one gel, and with a universal internal standard, multiple gel can be run and analyzed at the same time. 2D-DIGE can be applied to protein expression profiling as well as comparative proteomics using 2D analysis software to identify the proteins with a statistically significant difference in expression. 2D-DIGE provides a wide range of opportunities for researchers in Cardiovascular diseases, Cancer and other related fields; the 2D-DIGE analysis can uncover important proteins associated with these conditions


MudPITMultidimensional Protein Identification Technology (aka, MudPIT) is a liquid chromatography based proteomic technique used for separating and identifying complex proteins and peptides when the interest is to identify all the proteins in the biological sample is analyzed. The peptide mixture is prepared and pre-fractionated on or offline using strong cation exchange followed by online reverse phase LC-MS/MS for peptide and protein identification.  The data is then searched against a species-specific database for protein identification.


iTRAQ Isobaric tags for relative and absolute quantitation (iTRAQ) is a proteomic technique that utilizes multiplexed isobaric chemical tags which allows multiplexing of up to eight biological samples followed by LC-MS/MS.   The iTRAQ reagents consist of an amine reactive group, a reporter group, and a balance group. Simultaneous protein identification and quantitation are possible when the iTRAQ labeled peptides are analyzed by LC-MS/MS.  The labeling preserves post-translational modifications (PTMs) allowing for PTM analysis as well.


TMT Tandem mass tags, similar to iTRAQ, allow simultaneous protein identification and quantitation but have a higher plexing power.  Using TMT allows a researcher to multiplex up to 16 biological samples.


Luminex xMAP Luminex xMAP (Multi-Analyte Profiling) technology is designed to simultaneously detect multiple targeted proteins including cancer markers cytokines, growth factors chemokines, etc.., in the same sample. This technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Featuring a flexible, open-architecture design, xMAP Technology can be configured to perform a wide variety of protein or nucleic acid assays quickly, cost-effectively, and accurately. xMAP Technology enables multiplexing of biological tests and produces results that are more efficient and comparable to traditional methods such as ELISA, western blotting, PCR, and traditional arrays.  The Luminex xMAP is an open source platform so there are many commercially available kits available or a custom analyte panel can be developed.

The choice of your protein identification method depends on your sample type, project goals, and budget…. At ITSI-Biosciences, we pride ourselves in the provision of standard and state-of-the-art proteomics services and have a wide array of bioanalytical kits for your proteomic and protein testing projects.

ITSI-Biosciences offers comprehensive bio-analytical solutions, accurate results done at a fast turnaround time. Contact us today for a quote and make your protein analysis stress free. You can reach us via Email;, Phone; 814-262-7331, or Fax; 814-262-7334.


Posted on

Cancer is an abnormal growth of cells which has the potential to spread to other parts of the body and destroy normal body tissue. Cancer is a major threat to health representing the second largest cause of mortality in the world following cardiovascular diseases, but thanks to advancement in cancer screening and treatment, survival rates are constantly increasing for different types of cancer. The etiology of cancer is still being investigated worldwide. In general, most cancers associated with DNA mutations, where the mutation can cause a healthy cell to allow rapid cell growth, fail to stop uncontrolled cell growth, or make mistakes when repairing DNA errors.  Age, habit, family history, environmental factors, lifestyle, certain health conditions, including ulcerative colitis are common factors known to increase the risk of developing cancer. According to the WHO, the number of global deaths is expected to rise by 45% between 2008 and 2030, and by 2030 between 10 million and 11 million cancers will be diagnosed each year.

Dating as far back as the 1900’s mass spectrometry is one of the most widely used analytical methods in biosciences and medical research. Mass spectrometry-based proteomics was developed as a primary tool for the identification of medically relevant biomarkers for disease advancement and analyzing response to treatment with better results for patients. Since its advent, this analytical technique has advanced steadily, being utilized in various applications ranging from forensic toxicology to cancer diagnosis.

Several scientific papers published in 2020 including those by Uzzaman et al [1] and Sun et al [2] describe the use of mass spectrometers in cancer research. Also, Liu et al [3] used a combination of MS-intensive methods such as isobaric tags for relative and absolute quantitation with two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2D LC-MS/MS) and 1D-targeted LC-MS/MS, on serum samples from healthy people (normal control, NC), patients with benign diseases (BD), and Pancreatic Cancer (PC) patients to identify novel biomarkers of PC. They identified more than 1000 proteins, verified 142 differentially expressed proteins, and finally targeted four proteins for absolute quantitation in 100 serum samples. The novel biomarker panel of apolipoprotein E (APOE), inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3), apolipoprotein A-I (APOA1), apolipoprotein L1 (APOL1), combining with CA19-9, statistically-significantly improved the sensitivity (95%) and specificity (94.1%), outperforming CA19-9 alone, for the diagnosis of PC. Radu et al [2] reports that mass spectrometry provides a powerful and sensitive approach for the analysis of biological systems, and dedicated instruments are available for different classes of analytes, such as proteomes, metabolomes, genomes, transcriptomes, etc.

ITSI-Biosciences offers comprehensive mass spectrometry services for cancer research. Specifically, services such as protein identification, protein quantitation, biomarker discovery, global protein expression profiling and post translational modification (PTM) mapping can be applied to all areas of cancer research. ITSI has been offering such mass spec services since 2005. Hundreds of human and non-human samples have been analyzed and thousands of proteins and candidate biomarkers have been identified by mass spectrometry. As little as 1ug of total protein can be analyzed. There are several complementary technologies including Agilent Bioanalyzer, Luminex xMAP and GE 2D-DIGE platforms are available at ITSI to support mass spectrometry-based cancer research. Figure 1 is a generalized ITSI LC-MS/MS workflow which has been applied to the study of several cancers [4-9].

Figure 1: Generalized workflow for biological sample analysis by liquid chromatography – tandem mass spectrometry (LC-MS/MS) at ITSI. Post translational modification mapping (PTM) and gene ontology classification are additional services that add value to the data obtained by mass spectrometry.

ITSI Biosciences uses LC-MS/MS ion trap mass spectrometry technology.  All biological sample types can be analyzed at  ITSI-Biosciences, including 1D-gel slices, 2D gel spots, protein lysate, blood, serum, plasma, saliva, urine, whole tissue, western blot membranes, formalin-fixed paraffin embedded samples and laser micro-dissected samples. The standard mass spectrometry workflow may involve processing samples in a specialized way to isolate proteins, the proteins are reduced and alkylated. The samples then go through a cleanup step using our in-house ToPREP total protein precipitation kit.  The precipitated samples are resuspended in a digestion buffer and digested with trypsin or other enzyme as needed to achieve the goals of the analysis. The digested peptides are then separated by an inline liquid chromatography step at a nanoliter flow rate, to separate the peptides by reverse phase chromatography on a C18 column prior to an electrospray ionization step.  The ionized peptides are then analyzed using a high performance LTQ XL or Q Exactive orbitrap mass spectrometer.  The raw data files are further processed using Proteome Discoverer to identify peptides, proteins, and common peptide modifications. Additionally, Gene Ontology can be performed to define the molecular functions, cellular locations and biological process that the identified proteins may carry out.

The typical turnaround is 1-2 weeks depending on the project. For more information, contact ITSI-Biosciences via Email;, Phone; 814-262-7331 or Fax; 814-262-7334.


  1. Uzzaman A, Zhang X, Qiao Z, Zhan H, Sohail A, Wahid A, Shang Z, Guan X, Cao CX, Xiao H (2020). Discovery of small extracellular vesicle proteins from human serum for liver cirrhosis and liver cancer. 2020 Aug 21:S0300-9084(20)30198-X. doi: 10.1016/j.biochi.2020.08.013. Online ahead of print.PMID: 32835735
  2. Sun S, Zhang H, Wang Y, Gao J, Zhou S, Li Y, Han S, Li X, Li J (2020). Proteomic Analysis of Human Esophageal CancerUsing Tandem Mass Tag Quantifications. Biomed Res Int. 2020 Aug 7;2020:5849323. doi: 10.1155/2020/5849323. eCollection 2020.PMID: 32832552
  3. Liu X, Zheng W, Wang W, Shen H, et al (2017). A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. Br J Cancer. 2017 Dec 5; 117(12): 1846–1854. doi: 1038/bjc.2017.365.
  4. Radu Albulescu, Andrei Jose Petrescu, Mirela Sarbu, et al (2019). Mass Spectrometry for Cancer Biomarkers, Proteomics Technologies and Applications, Ibrokhim Y. Abdurakhmonov, IntechOpen, DOI: 10.5772/intechopen.85609. Available from:
  5. Somiari RI, Sullivan, A, Russell, S, Somiari, S, Hu, H, Jordan, R, George, A, Katenhusen, R, Buchowiecka, A, Arciero, C, Brzeski, H, Hooke, J, Shriver, C. (2003). High throughput proteomic analysis of infiltrating ductal carcinoma of the breast. Journal of Proteomics. 10 (3): 1863 – 1873
  6. Somiari, RI, Somiari SB, Russell S and Shriver CD. (2005) Proteomics of breast carcinoma. J. Chromatography B, Analyt Technol Biomed Life Sci. 815: 215-225.
  7. Boyiri T, Somiari RI, Russell, S, Aliaga, C and El-Bayoumy K (2009). Proteomics of rat prostate lobes treated with 2-N-hydroxylamino-1-methyl-6-phenylimidazo [4,5-b] pyridine and 5α-dihydrotestosterone. International Journal of Oncology 35: 559-567.
  8. Bortner J; Das A; Umstead T; Freeman W; Somiari R; Aliaga C; Phelps D; El-Bayoumy K (2009). Down-Regulation of 14-3-3 Isoforms and Annexin A5 Proteins in Lung Adenocarcinoma – Induced by the Tobacco-Specific Nitrosamine NNK in the A/J Mouse Revealed by Proteomic Analysis. Journal of Proteome Research 8(8): 4050-4061
  9. Arciero C, Somiari, S, Shriver C, Brzeski, H, Jordan, R, Hu H, Ellsworth D, Somiari, RI (2003). Functional relationship and gene ontology classification of breast cancer biomarkers. International Journal of Biological Markers 18 (4): 241-272.

Genplates Technology


Posted on

GenPlates are an automation friendly, high density GenSaver paper-based device that allows biobanking of blood and other biological fluids in a 384-well format. GenPlates are now the preferred technology for collection and long-term storage of DNA in Dried Blood Spots (DBS) samples and are ideal for collecting samples in areas where cold storage is unavailable, inconsistent due to power failure or expensive. It stores samples in the dry-state and at ambient temperature and provides high-level protection of samples during shipment. This technology helps eliminate the burden involved in storing samples cold using wet-ice or frozen with dry-ice, thereby reducing the cost of biosample acquisition, transportation, and storage.

GenPlates have been in existence and employed for the storage and protection of DNA in dried blood spots since 2006, and the technology is protected by US and International Patents. This technology provides long term, high-density storage of blood, blood products, and biosamples.

The study by Somiari et al (2011) titled “STR Analysis of Human DNA Samples After Dry-State Ambient Temperature Storage in GenPlates”, demonstrates that an average of 32.2% and 4.9% more DNA was recoverable per ul blood from GenPlates compared to frozen blood after two weeks and 16 months storage dry state storage, respectively. This result apparently demonstrates that higher amounts of DNA are recoverable from GenPlates per microliter of blood compared to blood samples stored frozen.

Each GenPlate is in a 384-microplate format and wells contain a 6-mm disc of GenSaver paper. There are 5 different configurations that allow the collection of different sample aliquots to fit the purpose. For example, the 1 – region plate configuration allows the collection of 384 unique samples/plate, one aliquot each or 384 different samples/plate, whereas the 24-region plate configuration allows the collection of 24 different samples in 4 aliquots. The GenPlate has the following specifications:

  • Each well of the sample region contains a 6mm disc of GenSaver paper
  • Each disc in the well will hold 10µl of whole blood, purified genomic DNA (gDNA), purified plasmid DNA (pDNA), crude bacterial suspension (clones), or glycerol bacterial stock
  • Using GenSolve each disc yields approximately 100ng of DNA
  • Each plate is foil sealed top and bottom
  • GenPlates are formatted into five different configurations
  • Each plate contains a 14-digit barcode which specifies plate type, region configuration, and unique plate number.
  • GenPlates are available in various formats to suit your research and DBS storage needs

GenPlates are manufactured by GenTegra, LLC. ITSI-Biosciences is the sole distributor of GenPlates globally. For more information, contact us via Email:, Phone: 814-262-7331 or Fax: 814-262-7334

ITSI- Business Manager

Posted on

ITSI seeks a Business Manager in Johnstown, PA, responsible for managing all of the financial aspects of ITSI-biosciences’ business, including preparing and maintaining all accounting and financial records to ensure accuracy and legal compliance as well as monitoring project costs, approving expenses, managing customer project accounts and ensuring timely payment collections. specific duties include: (i) reporting directly to the chief scientific officer on financial and sales tax processes as well as project management activities/project progress; (ii) liaising with SAP&DC for international business growth as well as external project stakeholders to achieve business goals; (iii) managing procurement and sales contracts as well as logistics activities; (iv) preparing financial statements, budgets, and cost/sales forecasts related to project incomes and expenditures; (v) managing the advertisement of ITSI’s product and service offerings to improve business awareness; (vi) implementing internal controls for accurate financial reporting and risk management initiatives; (vii) preparing and submitting business tax returns and government reports; (viii) tracking and managing project related incomes and expenditures; (ix) reviewing accounting classifications and creating new accounts to properly reflect actual transactions; (xii) managing the performance of international distributors and representatives; (xiii) negotiating contracts with external vendors to reduce cost and improve company profitability; (xiv) ensuring the timely delivery of projects in alignment with financial targets; (xv) preparing and maintaining monthly business management accounts, leases, and depreciation records; (xvi) managing employee scheduling activities and project task distributions; (xvii) preparing monthly project management accounts, reviewing and evaluating cost variances; and (xviii) identifying and implementing financial and accounting process improvement tools /method.

Must have a bachelor’s degree in Accounting or Business Administration.

Must have two (2) years of experience working in the Accounting or Business Management fields, including two (2) years of experience with each of the following: (i) overseeing the accounting and finance-related functions of a business; (ii) managing and updating fixed asset register and posting transactions; (iii) performing monthly reconciliation and reporting on financial accounts; (iv) providing variance analysis on income and expenditures; (v) reviewing and updating finance procedures; and (vi) producing annual statutory accounts.

Send resume to:

Inheritance 101: How Your Genes Make You YOU

Posted on

Most people have a working knowledge of genetics. Many of us learned about the basics of genetics and inherited traits back in middle school, but fail to remember the ins and outs of how it works. You might blame genetics for your crooked teeth or a bad back, but there are countless ways that genetic material works together to make you who you are.

Want to learn more about genetics? Keep reading to see how you came to be.

What is the study of genetics?

The study of genetics involves researching genes and DNA in order to figure out which traits and health issues are passed down through a family. These inherited traits can be good or bad depending on how you look at it; for some people inheriting their mother’s blue eyes can be a cool genetic trait. For others, inheriting a medical condition or gene, like the anti smith antibody, can foretell the presence of certain diseases.

By studying genetics, we can answer a few separate questions, namely why we look the way that we do and what might happen to our health down the line. For example, some people are a carrier for mutated genes that can lead to health problems in the future. On the other hand, prenatal genetic testing can help parents better prepare for inherited genetic abnormalities, such as Down syndrome. Through simple blood testing, we can also determine if a person carries abnormal diseases as a result of a mutation, like the presence of the anti smith antibody.

How are genes passed down?

Your DNA is made up of tiny, winding structures called chromosomes. It’s within these structures that your genetic information resides. Humans are born with 46 chromosomes that exist in 23 pairs; each half of the pair is randomly selected from a parent.

It’s thanks to these unique combinations of chromosomes that researchers are able to glean information about a person. Thanks to DNA collection that the Combined DNA Index System (CODIS) contains profiles of more than 16 million convicts and over 750,000 crime scenes. By matching DNA patterns, investigators are able to determine if a person is innocent or guilty.

Dominant vs recessive genes

Genetic researchers are most often interested in genetic abnormalities and inherited diseases. Amidst the copies of chromosomes for each parent, you have a chance to inherit a chromosomal abnormality. However, this doesn’t always happen. For example, if you have one parent with an inherited dominant disease, there is a 50% chance you might inherit the gene that caused the disease as well.

A recessive gene, on the other hand, is a little different. Should both parents have copies of a genetic abnormality, there is then a 25% chance that it might be passed onto you, even if both parents don’t develop symptoms of an illness. This would make the parents a carrier of the disease. For example, should both parents have the anti smith antibody, there’s then a 25% chance that you might inherit it.

The field of genetics is rife with developing technologies. When you want to learn more about yourself and your loved ones, don’t hesitate to reach out to ITSI Bio for more information.

What Advantages Does Noninvasive Prenatal Sampling Offer You?

Posted on

Noninvasive prenatal sampling, also known as noninvasive prenatal testing, can offer new parents a variety of benefits. This harmless test is performed by doctors and DNA testing services across the country in order to determine genetic abnormalities in the fetus. All the mother needs to do is offer a small sample of blood for DNA seq analysis to occur.

This is because the mother’s blood contains vital information about the placenta, and therefore, about the pregnancy. By checking the information in placental cells, doctors and DNA analysts are able to determine the cellular makeup of the fetus since these components are usually identical.

If you’re interested in noninvasive prenatal sampling, here’s how this beneficial process can help you.

It can prepare the parents for the future

As a prenatal test, this kind of procedure is inherently designed to better help a parent prepare for the future. This kind of test can offer a slew of information to the parents. For example, it’s a great way to determine the sex of the fetus and it can even indicate its blood type. Should there be a complication during pregnancy and donor blood is needed, this kind of information can be vital to saving an infant’s life.

Noninvasive prenatal testing is also beneficial to detect genetic abnormalities. It’s not uncommon for older parents to birth babies with genetic differences. When a mother is over the age of 35, they experience an increased likelihood of birthing a child with trisomy differences, such as trisomy 21 — also known as Down syndrome — trisomy 18, and trisomy 13. Countless families can better prepare for the needs of their child when they know that such differences are present.

It’s more accurate than other maternal tests

In the past, we relied on maternal serum tests to offer this information. While also considered noninvasive, these tests are far less accurate than NIPS. For example, it’s estimated that one in 20 mothers would get a false positive regarding a Down syndrome diagnosis when they used a maternal serum. By contrast, NIPS will result in a false positive one in every 1,000 mothers.

It doesn’t harm the fetus in any way

The noninvasive aspect of this test means that the fetus will not be harmed. By taking a simple blood test from the mother, which contains both her cells and placental cells, analysts are able to draw conclusions without impacting the fetus whatsoever. You can rest assured that the fetus will be fine when engaging in this type of test.

Whether you’re looking for noninvasive prenatal sampling or protein testing analysis, rely on the experts at ITSI Bio to help you better prepare for your future.

Everything You Need to Know About DNA Profiling

Posted on

DNA profiling, formerly known as DNA fingerprinting, is a chemical test that aims at showing the genetic makeup of a person. The tests help in identifying a person and his or her kin.

Nowadays, criminal agencies are using technology to identify criminals and solve crimes. The technology is also used for proving or disapproving specific consanguinity claims.

Basic Information about DNA Profiling

The process uses specific tools like a buccal DNA collection kit to gather samples from a person. It is worth noting that in this case, only a small amount of buccal DNA is needed for analysis. For example, 100 mcg of sample DNA is enough to map the subject’s biological information. That means a splotch of saliva on a drinking straw is more than enough for DNA sequence analysis.

After taking the correct sample size, the DNA testing services amplify significant regions. Experts use the polymerase chain reaction (PCR) to create copies of these regions with Short Tandem Repeats (SRTs). The SRTs vary from one person to the other.

After amplifying the STRs, the repeats are then counted. Here, the experts attach fluorescent dyes onto the STR copies. The mixture is then run through lab equipment known as capillary electrophoresis machine to size different DNA fragments. After identifying the size of each repeat, it becomes easier to identify the repetitive unit count and length of each STR.

After doing all this, the next thing is looking for matches. If any person’s STR matches that of the sample in 13 STR regions, there is a match. So, if this was an accused person, the courts may rely on this evidence and convict the person. The margin of error in these tests is one in a billion samples. Due to its accuracy, criminal cases have been relying on DNA profiling for more than 30 years now.

Here are the primary benefits of this technology.

1. DNA Profiling Is Less Intrusive

Sampling someone’s DNA doesn’t require big samples. Although a buccal DNA collection kit is needed, only a small amount of biological footprint is necessary to map and match the DNA.

Likewise, in crime scenes, the biological footprints may be invisible to the naked eye, making it impossible to identify the person. But not anymore. The hair strands, smudges, blood, and saliva are enough for DNA seq analysis.

2. Helps Reduce the Conviction of the Innocent

Using DNA profiling helps reduces cases where innocent people are convicted. It is even more reliable when other forensic evidence and tools are in use. The forensic team will collect samples using a buccal DNA collection kit and reference it against the current data. Such a simple process can mean the difference between freedom and jail.

3. Assists in Solving Crimes

Samples of DNA obtained through buccal DNA collection kit or any other collection tool remains valuable evidence for a long time because the DNA is a tough and flexible molecule which does not denature with ease.

With this, the authorities can use DNA evidence collected from old crime scenes.

4. Identifying Victims of Catastrophic Events

At times, some accidents like fire breakout and airplane crashes can destroy a body beyond recognition. In such cases, the DNA testing service will use buccal DNA collection kit on a surviving family member to map it against that of the deceased. Such an exercise is important in providing closure for the family.

5. DNA Emphasis Has More Than Criminal Emphasis

People can use DNA sampling and profiling in different ways to better their lives. For instance, DNA profiling can help people determine their ancestry, lineage, and heritage. The information is also useful in determining biological parentage.

An analysis will also identify people who are at risk of developing genetic diseases and cancers. All this helps one to start treatments on time effectively reducing the chances of death.

Except for identical twins, two people cannot share the same DNA pattern. Therefore, people and investigating authorities can rely on DNA evidence to direct or conclude criminal cases. Taking DNA samples from a crime scene allows investigators to focus their time and energy in the right direction.

5 Examples of Services ITSI Biosciences Provide

Posted on

ITSI Biosciences supports many laboratories, educational institutions, municipalities, and medical practices for a variety of purposes. Here are five examples of services ITSI Biosciences provides:

Research Support

Biomedical research has exploded over the past few decades and the number of jobs in the biomedical field is estimated to grow by over 20%. ITSI Biosciences supports researchers with products to collect and analyze samples, including buccal DNA collection kits, sample tubes, reagents, and lab equipment.

For researchers who do not have access to the labs or the lab equipment necessary for their research, ITSI Biosciences provides testing services. Researchers can rely on ITSI Biosciences’s labs to follow laboratory best practices and even aid in the design of experiments to obtain the precise experimental data sought. By outsourcing testing and analysis, researchers can focus on the theoretical and academic side of their research.

Laboratory Support

State and local police labs, university labs, and medical labs often lack the time, capacity, or equipment needed to perform all the testing and analysis needed. As lab workers know, testing and analysis take time and personnel and it is impossible to perform rush jobs when the reactions require a minimum amount of time. Moreover, cutting corners or performing sloppy work to try to rush through testing and analysis can lead to unreliable or unusable results. ITSI Biosciences can take the pressure off laboratories with its state of the art equipment and trained laboratory professionals to perform the testing and analysis those laboratories cannot perform in a timely manner.

Protein Testing Services

Protein analysis is becoming an important diagnostic and prognostic tool. ITSI Biosciences provides protein analysis to identify proteins that have been correlated to many illnesses and conditions. For example, protein analysis has been used to aid in the diagnosis of prostate cancer, ovarian cancer, tuberculosis, and severe acute respiratory syndrome (SARS). Moreover, protein analysis can serve as a prognostic tool, with research suggesting that analysis of proteins may identify the severity of organ rejection in transplant patients and the likelihood that tumors will resist specific drugs and treatments in cancer patients.

DNA Testing Services

DNA analysis has been used for a variety of purposes in research and forensics. ITSI Biosciences’s DNA analysis can provide scientific evidence that determines whether samples from an individual correlates with samples collected in the field. This type of analysis is used most frequently in two areas. Samples collected from an individual can be compared to samples collected from disasters or mass casualty crime scenes to identify victims. Similarly, samples collected from an accused individual can be compared to samples collected from a crime scene during a criminal investigation to identify perpetrators. ITSI Biosciences performs these types of analyses objectively to produce evidence that can be used to exonerate or incriminate accused individuals.

DNA analysis is also useful for providing scientific evidence to determine whether a familial relationship exists between individuals. As with individual identification, relational identification can be used to establish or refute paternity, maternity, or other familial relationships in such cases.

Aside from its evidentiary use to identify individuals or determine familial relationships, DNA analysis is also used to classify the species or lineage of plants, animals, and microbes. For example, DNA analysis can assist researchers in differentiating between strains of infectious diseases, establishing relationships within animal populations, or investigating evolutionary paths of organisms.

RNA Analysis

Like protein analysis, RNA analysis is proving to be an important diagnostic tool for genetic diseases. ITSI Biosciences can provide RNA analysis to identify genes that code for enzymes, antibodies, and other proteins associated with biological processes like viral response, immune response, and cellular activity.

Moreover, genetic diseases associated with Mendelian traits, such as cystic fibrosis, sickle-cell anemia, and Tay-Sachs disease can not only be diagnosed but can be predicted by testing both parents before conception. Genetic diseases that are non-Mendelian can be researched by sequencing RNA to attempt to identify the processes that lead to inheritance of the non-Mendelian trait.

ITSI Biosciences can support researchers in designing and executing experiments to collect the data needed for their research. Similarly, ITSI Biosciences can provide the capacity and capability to support laboratories in their testing and analysis. These testing and analysis services include experimental, diagnostic, prognostic, and forensic uses of DNA, RNA, and proteins.

3 Guidelines to Follow When Collecting Forensic DNA

Posted on

DNA was first used as a forensic tool in the United States in 1988 but its roots can be traced back to the origination of biotechnology originated in 1919. But what is DNA analysis?


DNA analysis, when used as a forensic tool, uses “short tandem repeat” (or STR) analysis. By counting the number of repeats of short pieces of DNA at each of those locations, a statistically unique DNA profile can be determined for each individual. In the United States, the FBI’s Combined DNA Index System (or CODIS) examines STRs at 13 different locations, which gives a theoretical probability of a false match of about one in one quintillion. However, false matches do occur in practice, mostly due to contamination in the collection, storage, and testing processes. Here are three sources of guidelines that attempt to minimize the risk of contamination of forensic DNA:


International Organization for Standardization


The International Organization for Standardization (or ISO) prepares standards for a variety of industries. ISO 18385:2016 is the standard for manufacturers of products used to collect, store, and analyze forensic DNA. Rather than addressing the question, “what is DNA analysis,” the primary goal of the ISO standard is to minimize the risk of contamination at the manufacturing source. The standards are rigorous in the handling of the materials that can cause the inadvertent transfer of DNA from, for example, a factory worker to the latex gloves or cotton swabs made at that factory.


This is not a theoretical problem. In the late 1990s and early 2000s, police in Germany, Austria, and France collected DNA evidence from over 40 crime scenes, including six murders, that all had the same female’s DNA profile. They named the serial criminal the Phantom of Heilbronn due to the disparity in the crimes, locations, and descriptions of the perpetrator.


But in 2009, police realized that the DNA profile they had developed had come not from the perpetrator of those crimes. Rather, it came from a factory worker where the swabs were produced. The swabs had become contaminated with the factory worker’s DNA and had not been treated to destroy the contamination before the assessment took place. The factory’s mistake was that, although the swabs were sterile (meaning they were not contaminated with bacteria and viruses), the swabs were not purged of DNA, which survives sterilization. ISO 18385:2016 sets out the processes and procedures that manufacturers can take to ensure that products used to collect, store, and analyze forensic DNA are “DNA-free.”


National Institute of Standards and Technology


The U.S. National Institute of Standards of Technology (or NIST) has studied the handling of biological evidence and issued a handbook that outlines their recommended practices for collecting and handling biological evidence. This includes samples that will be subjected to DNA testing services and DNA seq analysis. Like the ISO standards, the best practices handbook from the NIST begins by answering the question, “What is DNA analysis?” and proceeds through the steps of collecting, packaging, handling, storing, tracking, and destroying biological evidence subject to DNA analysis. The NIST handbook also includes guidelines for safe handling practices by evidence handlers to minimize the risks associated with the exposure to potentially hazardous or infectious biological samples. These guidelines essentially follow the OSHA standards.


American Bar Association


The American Bar Association (or ABA) has also issued DNA evidence guidelines in response to the question, “What is DNA analysis?” As might be expected, these guidelines focus on the collection, testing, handling, storage, and destruction of DNA evidence from the perspective of its use in criminal proceedings. The ABA does not take sides in its guidelines but rather seeks to ensure that the evidence is collected legally and in a manner that makes it useful in a subsequent criminal trial. The ABA guidelines include guidelines for collecting DNA from crime scenes as well as from people, such as through the use of a buccal DNA collection kit. The ABA guidelines also address the standards for testing laboratories and their lab equipment.


For more information on DNA analysis and the best practices used in forensic work, rely on ITSI BioSciences today.

The Importance of Biotech Advancement

Posted on

The roots of biotechnology go all the way back to 1919, but it has not stopped growing and changing as the years go on. In fact, it only took 50 years for researchers to perform the first successful experiment with recombinant DNA. The technology and knowledge available in this field continues to expand, even today.

These advances can be seen in all areas of the field, including DNA seq analysis, mass spectrometry protein analysis, and protein testing services. With so much innovation happening in a variety of fields, this means that biotech companies have to keep adapting. New lab equipment and testing approaches are meant to make the field more productive and easier to manage. After all, it is a crucial field when it comes to the advancement and health of the human race. Below is a closer look at the importance of growth in biotechnology.

Advances in Healing

One of the greatest benefits of biotechnology and DNA seq analysis is that it’s helping lead the world to better healthcare. Learning more about how the human body works and how different diseases form and develop enables advancements in diagnosing and treating those diseases. In some cases, these studies can even help to prevent the disease from infecting people in the first place. Creating medications, vaccines, and other medical approaches must start with a solid understanding of the human body and how it can get sick. The ability to offer DNA testing services is a huge leap in healthcare technology on its own.

Advances in Agriculture

Biotechnology doesn’t stop with the human body. It also branches into the world of agriculture. This field of study is helping change the way that farmers do their job. With the help of biotechnology, it’s possible to come up with safer and more environmentally friendly ways to grow crops. Researchers can even help farmers save money by finding different ways to run their farm that don’t take up as much energy and natural resources.

Advances in Knowledge

Understanding the world around us and how it works is very important when it comes to making smarter decisions for the planet — and ourselves. Whether it’s monitoring diseases or investing in new ways to grow crops, advancing the field of biotechnology means advancing other fields as well. As the biotechnology field continues to grow and develop any number of opportunities can unlock ancient truths or new ways of living. For more information about biotechnology, DNA seq analysis, or agricultural advancement, rely on the help of ITSI BioSciences today.

Why Biomedical Testing is a Great Career Choice

Posted on

Biotechnology is a crucial part of modern medicine and science. The ability to perform tasks such as protein testing services and DNA testing services allows humans to learn more about themselves and the world around them. Identifying the presence of the Anti Smith antibody (anti-Sm antibody) can help to diagnose specific diseases. The use of lab equipment is helping uncover cures for diseases and make breakthroughs about the human body as well as the Earth.

When one considers all of the benefits that the biomedical field has to offer, it’s clear that biomedical testing is a career that is worth considering. It offers an opportunity to help others and to learn more about the way the human body functions. If you have a love for science, here are some reasons why you might want to consider a position in the biomedical field as a career.

Job Growth

The projected growth for the biomedical field is currently estimated at 23%. That’s much higher than the average that sits below 10%. This means that there is likely to be jobs available in the field when you finish with your schooling and that you will be able to make something of the time and money you spend on college. In today’s economy, that’s not something every field can offer.

There is also room for growth within the position. The biomedical field is complex and you have room to move up into new and more exciting positions. With the right knowledge, a love for science, and curiosity about anti-Sm antibodies, you could go far in the field.

Help Others

Biomedical testing is a great way to help others. DNA testing and protein testing can help people learn more about their bodies and their health risks. You can help to diagnose someone with a disease so that they can get the help they need or allow someone to access preventative care for a disease they might be more likely to end up with.

Understanding the biology of the world and helping others understand their own biological makeup are great ways to help people. You can assist in saving lives and be a part of the progression of the scientific world.

Great Pay

Becoming a biomedical engineer isn’t an easy feat and the work takes great skill. This means that it also pays incredibly well compared to your average 9 to 5 job. Most people can’t tell you what an anti-Sm antibody is, but a biomedical engineer can. It’s that in-depth scientific knowledge that sets them apart and earns them a higher salary. If you’re looking for a career that is as lucrative as it is interesting, biomedical testing could be a great choice.

Common Uses Of DNA Testing

Posted on

What is DNA Testing?

DNA Testing Services (also referred to as protein testing services) have been getting more and more attention lately. With large companies like and 23andMe, it’s no wonder people are curious about DNA testing services. But just what can you learn? What is DNA analysis and how does it work? Let’s explore some of those questions now.

DNA testing is a method that takes samples of a person’s DNA, which could be their hair, fingernail, skin, or blood, to analyze the structure of that person’s genome. DNA testing can help establish parentage (or lack thereof), ancestral history, and even help police investigate a crime scene.

Regardless of what DNA testing services are used, at the very heart of the analysis is DNA itself: Deoxy-Ribonucleic Acid. DNA is a self-replicating molecule that is the foundation of what keeps people moving, living and breathing. DNA is what makes you unique.

Uses of DNA Testing Services

What are some of the main uses of DNA Testing Services? DNA testing can be used for a variety of things. Though it requires very specific lab equipment, a DNA SEQ analysis can be done relatively quickly and at a much lower price than might be expected. Here are some reasons you might decide to make use of DNA testing services:

  • Archaeology: That’s right, Indiana Jones would be proud. Archaeologists now use DNA tests to keep a genetic record of the progression of life throughout the centuries. This helps us maintain a better genetic record to understand where ancient people lived, and which ancient groups interacted with each other. Archaeologists can even use this to explore migration patterns of different groups of ancient humans.
  • Paternity Testing: This is probably one of the most common things people think of when they hear, “DNA Testing Services.” Using DNA is the most definitive way of confirming who a child’s parents are. This could be done for legal reasons or simply out of curiosity.
  • Finding Genetic Ancestors: Understanding where we come from has been an important part of our desire to belong. Because of the large amounts of interest people have had in genealogy over the last few decades, DNA tests can now be used to help a person understand where their genetic heritage lies. Understanding ethnic origins of ancestors can be fun, but it also helps us realize just how connected all humans are.
  • Prenatal Genetic Tests: DNA testing services also play a more practical role in medicine today. Doctors can now use DNA testing to assess an unborn fetus for health problems. DNA testing can be used to estimate the probability and risk of certain genetic diseases. In addition to this, DNA testing can also be used to help identify cancer in its early stages, helping doctors and patients alike get critical care and make lifestyle changes necessary to mitigate cancer risks.
  • Criminal Investigations: As mentioned previously, using DNA to help investigate the scene of a crime isn’t just for the movies. DNA tests are commonly used in criminal investigations today. In addition to helping solve crimes that were recently committed, DNA testing has also been used by the police to reopen past cases that went “unsolved” because of the lack of evidence. DNA testing is changing the game for our men and women in blue, as it gives them a new way to analyze evidence.

DNA Testing is only just beginning

It doesn’t matter what your interest in DNA testing is, there is a reason to get involved. DNA testing is still in relative infancy compared to other applied sciences. In fact, jobs in the biomedical field are expected to grow by 23%. As it continues to advance, the applications for DNA testing services are expected to grow more and more diverse, helping police forces, medical professionals, and everyday members of the human community.

5 Tips For Buying The Best Lab Equipment

Posted on

If you are a lab professional, you know that buying the right lab equipment can be a challenging task when you do not know the things to consider. You need to ensure that you have the right tool for your work. Your customers will be expecting accurate results after analysis of their samples, and this depends on the tools that you use to do the work.

In addition to that, you want the kind of equipment that will help you to analyze samples faster. Even though there may be many types of equipment, a good professional knows that it is not wise to buy without verifying their suitability. Biotechnology dates back to 1919, but researchers had to wait for 50 years for the first successful DNA exercise.

Look at the brands and quality

Before you do anything, you need to collect information about some of the most preferable brands and models. There are brands that are known for their quality because the manufacturers have been supplying them for many years. These are the brands that you are likely to find in the biggest labs because their quality makes them the best choice. However, it does not mean that you cannot find new brands and models that are good too. You need to know all the information about so you are sure of the quality you will get when you buy things like DNA SEQ analysis equipment.

Check out the main features and specifications

You cannot buy lab equipment for protein testing services without looking at the features. Look at how they function and how much value they are likely to add to your lab. You need to also check the technical specifications to know the mounts of samples that they can test and how fast they do it. If you have the time, it will be better for you to compare these specifications and features from several products. At this point, you will be surprised to notice that even though they are meant for the same work, there is a big difference in their features.

Ask yourself how easy it will be to use the equipment

If you are experienced in lab work, you have an idea about the tasks that are easy to complete and those that are too demanding. When buying things such as mass spectrometry protein analysis, you need to know how much they will simplify your work. The purpose of buying equipment is to make your work more manageable, and therefore, you cannot afford to go for that which does not provide it. It is true that everyone wants equipment that has many features and lots of options; however, if it is going to make your work too complex, you may have to rethink your choices.

What is your budget?

Every business has a budget that they can afford to spend on buying lab equipment. Whether you are thinking about anti smith antibody or DNA testing services, you cannot go out shopping without knowing how much you can pay. Because of this factor, you should start by sourcing the funds. You will notice that big labs can afford more expensive equipment than small ones. In addition to that, the needs vary depending on the kinds of services that you offer. The good thing is that you can always find affordable equipment.

After sale services and warranty

You need to find out if the manufacturers will provide support for the equipment after you buy it. Mostly, they will agree to provide certain services for a certain duration. For example, they could offer to teach your lab workers how to make the best use of some machines. They may also offer to transport and fix any defaults that may occur during the first year. Although some of the services may seem minor; they could save you a lot of money.

If you want to find the best lab equipment, you should look for a supplier that is trusted. You can always buy these items either online or locally. The most important thing in this situation is to check if they stock the kinds of tools that will help you to improve the quality of services that you offer in your lab.

lab equipment

Monitoring Human Health As Gauged By Chronological Age Vs. Biological Age

Posted on

Human curiosity has pushed the pursuit of biomedical science incredibly far in recent years. That said, it’s nothing we haven’t been exploring for years. Biotechnology traces its roots back to 1919, but the burning question what is DNA analysis? was inaccessible for another 50 years until researchers performed the first successful recombinant DNA experiment.

From then on, mass spectrometry protein analysis, DNA testing services, protein testing services, and the lab equipment that makes it all possible advanced exponentially. These advancements have been illuminating because in accessing and beginning to understand the genetic makeup of human beings, we see glimpses into scientific possibilities that were once deemed impossible.

Among these discoveries is the human fascination with mortality. Or, to borrow a more romantic term, immortality. Now, we’re not talking about living forever, but certain research into genomics has brought to light possibilities of extending biological age.

Genomic sequencing pioneer Lee Hood has been researching this idea for a long time. In medical patients, he found that chronological age and biological age can be drastically different. These ages are ultimately connected with human health.

“If you are younger than your biological age, you’re in great shape. And the younger your biological age, the better off you are. If you’re older, you should do something to reverse it,” Hood said.

Much easier said than done, as these ages are anything but cut-and-dry. Hood goes on to explain how certain health issues can be related to higher biological age regardless of chronological age. He uses information gleaned by his health startup Arivale, which gauges biological age and uses the information to increase wellness and ultimately lower biological age.

“We check this against Arivale patients who had diabetes. They were six years older than their chronological age. Against cardiovascular disease, they were three years older, and against the people that most fanatically use the Fitbit, they were three years younger,” he said.

Exposing the connection between these health issues and higher biological age opens the door to take steps to help make people healthier. The technology is still in its infancy, but the ideas and intent are there to help better inform physicians, scientists, and other health care professionals. However, it’s important to note that technologies and advanced medical lab equipment aren’t being developed to replacement human physicians.

“It’s to make their relevant, actionable, knowledge democratized so that physicians can use that knowledge to make a difference for these patients really effectively,” said Hood.

Lab equipment, technological integration in medical processes, healthcare startups, data analysis, physicians, and patients are all in the process of forming a powerful alliance that will lead our society into a healthier future.

total protein assay kit

Looking for Protein Levels? Use Our Total Protein Assay Kit

Posted on

Testing is an important part of the medical field. It plays a crucial role in diagnostics. A total protein assay kit helps doctors analyze what is going on with their patients. 

Whether the doctor is conducting a routine health check, investigating unexplained weight loss or fatigue, or checking for kidney or liver disease, a protein test is necessary. The accuracy of the kit can make a huge impact on a patient’s life.

The right total protein assay kit varies from lab to lab. To see if ITSI-Bioscience protein assay kits work for you, learn more below.

The Lowry Method

The Lowry protein assay method has been around since the 1940s, thanks to Oliver H. Lowry. Out of all the important medical papers published throughout time, Lowry’s work on this method is the most cited.

The Lowry method is based on the reaction of copper ions to peptide bonds. Unfortunately, this method is affected by the presence of other chemical compounds in the mixture. 

Many of today’s protein assay methods are modified versions of the Lowry method.

The Bradford Method

Compared to the Lowry method, the Bradford method is newer, developed in 1976 by Marion M. Bradford. This method is affected by the pH balance of the mixture, and depends on the amino acids of the proteins. 

With the Bradford method, dye binding is used to analyze results. This method is less susceptible to other chemicals present in the mixture, which makes is preferable to the Lowry method. Unfortunately, the amount of buffer present can affect the outcome. 

If a new assay method is not based on Lowry’s, it’s likely based on Bradford’s. 

New Methods

While the Lowry and Bradford methods are important, foundational scientific breakthroughs, some biochemists complain that they are outdated. Biochemists have to consider the content of their sample before deciding which is best.

Most labs prefer a kit where outcomes are less susceptible to their buffer system, that can analyze small samples, and aren’t affected by other chemical compounds that may be present.

ITSI-Biosciences Kits

The ITSIPrep Bradford Total Protein Assay Kit is based on the Bradford assay method but is optimized for better performance. Not only is it more sensitive than the traditional Bradford method, but it is more tolerant of most buffer systems.

Users find our kits are more consistent than the standard Bradford array and faster than the Lowry array. The results can be reproduced and the reagents are ready to use. 

These kits are cost-effective for medical labs, providing the lowest cost per sample compared to other kits. They are also easy to use, so there’s no need to use your best manpower. 

These kits include all quanti-protein and curve reagents, curvettes and microtiter plates, assay tubes, and an easy to use protocol. You can find this kit in two assay quantities: a cheaper one for 20 assays, and a good value for 96 assays.

Our Total Protein Assay Kit

The ITSI-Bioscience protein assay kit is constructed with convenience, available protein, various mixtures, and accuracy in mind. 

Is your lab is looking for an effective total protein assay kit? If so, don’t hesitate to contact us to learn more about our products. 

Protein Isolation Kit for Urine Samples

Cancer Diagnosis and More: The Different Uses of a Protein Isolation Kit for Urine Samples

Posted on

You don’t have to suffer for your health. 

If you’re anything like most Americans, the fear of painful tests prevents you from getting the help you need. 

If you feel that taking tests at the doctor’s office has to be invasive, let us tell you about a great way to avoid this.

One of the newest tests on the market provides you with the opportunity to diagnose your disease effortlessly. It’s called a protein isolation kit for urine samples. This small test is changing lives.

If you want to know how it can improve doctor’s visits, keep reading.

What Is the Protein Isolation Kit for Urine Samples?

The protein isolation kit for urine samples, also known as the Bence Jones protein urine test, checks your urine for multiple myeloma, a type of plasma cell cancer that weakens your bones. The test also detects lymphoma or cancer of the lymph nodes.

These diseases can have a variety of symptoms including thrombocytopenia, which is when your body doesn’t have enough blood cells to form blood clots. This causes excessive bruising and can lead to anemia, which causes severe lethargy.  

Why Was This Test Recommended?

Though each body is different and it’s hard to know exactly how a disease is working inside you, there are some common symptoms that show you might need a Bence Jones protein test.

These symptoms include:

  • High levels of calcium in the blood
  • A severely weakened immune system that leaves you susceptible to infections
  • Numbness, weakness, and pain in the limbs
  • Dizziness, disorientation, and confusion
  • Weakened bones that are prone to pain or breaking

How Is This Test Done?

There are 2 different versions of the protein isolation kit for urine samples.

The first test you take over 24 hours, and it requires that you pee into a cup over the entire 24 hour period. Your urine sample and urine bubbles must be refrigerated overnight and then sent to a lab. 

The simpler version of this test involves a small sample called UPEP, or urine protein electrophoresis. This test is more straightforward and only detects a small amount of protein, in this case, the Bence-Jones proteins. 

Then a protein analysis is performed to see if there are any medical problems. 

Are There Any Other Tests I Need?

If you’re getting the protein isolation kit for urine samples done, then you may need other tests and procedures to ensure you’re healthy. These tests include, but aren’t limited to, the following:

  • The complete chemistry profile- This test gives you an idea of kidney function and if the myeloma has spread.
  • Complete blood count CBC- This tells you the number of platelets and red and white blood cells in your blood.

When Do I Get My Results? What Do They Mean?

The standard waiting time for results can vary heavily between a couple of days and up to 2 weeks.

If there’s an excess of the Bence Jones proteins, in rare cases it may be Waldenstrom macroglobulinemia, but it usually means multiple myeloma. As stated above, it may also indicate that you have lymphoma. 

Want More Information About This Test?

The protein isolation kit for urine samples makes taking care of your health painless.

If this sounds like a test you need to order for yourself or someone else, contact us today

How to Choose the Best Quantigene Services for Fast and Accurate Results

Posted on

QuantiGene Plex is a powerful tool. The right QuantiGene service can aid drug discovery and development. It can be a great addition to your lab’s tools for clinical research. 

Notice we said the “right” QuantiGene service. How can you tell the difference between the best QuantiGene services and the ones you should avoid? 

That’s exactly the question we will answer in this guide. 

Read on to peruse our checklist for picking the right QuantiGene Plex service. 

QuantiGene Qualifications to Seek

We’re not going to play favorites when it comes to mentioning specific services. Instead, we’ll show you what to look for in a QuantiGene service. Once you know what to look for, you can make an informed decision for yourself.

Take a look, and begin your education. You’ll be a QuantiGene expert in no time.

Quantity of RNA Targets

The point of QuantiGene is quantity. The word factors into the name of the service itself. Thus, you want to guarantee you’re finding a QuantiGene service that can measure as many genes as possible.

A good QuantiGene service should have the equipment to measure up to 80 genes.

No RNA Purification Required

RNA purification has its uses. Involvement in branched DNA assay does not have to be one of them.

We see RNA purification as an extra, unnecessary step in the assay. Many QuantiGene Plex companies see it the same way. That’s why they offer their services without the need for RNA purification.

When a service does not require RNA purification, it can work with degraded tissues and directly with blood. All of these things simplify the workflow, making your research easier and clearer. 

Check the Inventory

You’re going to use your QuantiGene Plex assay to create pathway-themed and disease-themed panels. These are complex panels that require analysis of multiple genes. You can’t run a disease panel when you only have a few genes to validate against.

You choose should choose a service that has an in-depth inventory of validated genes. This will give you the most thorough assay. 

It’s reasonable to expect your QuantiGene Plex service have an inventory of thousands of validated genes, and not low thousands. We’re saying around 15,000 genes is the ballpark you want to be in. 


Finding a service that can create custom panels quickly is just good sense. You want a QuantiGene service that can be a true partner to your lab. If you need a custom panel, your QuantiGene service should oblige. 

Custom panels are always going to take longer than standard panels to create. You should expect a little extra wait time. We think within a couple weeks is a reasonable standard. 

Plex Your Lab’s Muscles

Forgive the terrible pun, but it points to a truth. If you choose a high-quality QuantiGene Plex service, your research will grow stronger. We hope this guide has given you insight into how to find that service.

A lot of your search should be about quantity. Can they measure a lot of targets, and do they have a large inventory? If the answers to those questions are both “yes” and they can do their work quickly, you’ve got a winner on your hands.

Check out our services for more in-depth help with QuantiGene technology. 

The Anti-SM Antibody: An Overview for Medical Professionals

Posted on

The human body functions in amazing ways to keep foreign invaders from entering. Upon detection, immune cells act to neutralize and remove pathogens.

They tag them as dangerous, endocytosis, phagocytosis, and pinocytosis. Blood proteins called antibodies essentially sniff out specific antigens, discerning between self and non-self particles. Then, they appropriately dispose of them.

Unfortunately, the body gets mixed up sometimes and begins to produce antibodies that don’t make this crucial discernment correctly. As a result, they attack healthy cells. We see this in autoimmune diseases, such as systemic lupus erythematosus (SLE).

SLE is a chronic inflammatory autoimmune disease characterized by inflammation that affects the skin, muscles, bones, heart, lungs, and peripheral nervous system. With this disease, keratinocyte, and monocyte apoptosis increases along with the Fas ligand expression in T and B cells and T cell necrosis.

Antibody research revealed that one of the most important nuclear antibodies for diagnosing autoimmune diseases in clinical practice is the anti-Sm antibody. Let’s look at the specifics on the anti-Smith antibody and its clinical importance regarding SLE.

The Anti-Smith Antibody

This antibody targets the following proteins that compose U1, U2, U4, and U5 small nuclear ribonucleoprotein particles:

  • B/B’
  • D1
  • D2
  • D3
  • E
  • F
  • G

In some individuals suffering from lupus, dead cells release the anti-smith antibody. It combines with anti-RNP antibodies to form a self-reactive B cell protein complex.

Why Is It Clinically Important in Diagnosing SLE?

The anti-Smith antibody is present in about 20-30% of patients. Most of these patients are Asian and African American.

A higher percentage of patients with SLE present with anti-RNP antibodies. However, doctors find that the anti-Smith antibody test more accurately diagnoses the disease because this antibody doesn’t tend to present in other rheumatological afflictions, whereas anti-RNP does.

When Is It Appropriate to Test for Anti-Smith Antibody?

The doctor should test for this antibody if they suspect that the patient may suffer from SLE. Signs and symptoms that may help you decide to test include:

  • Headaches
  • Swelling and pain in the joints
  • Serositis
  • Severe fatigue
  • Anemia
  • A butterfly rash
  • Unexplained hair loss
  • Raynaud’s phenomenon

You can also use this antibody test to help diagnose mixed connective tissue disease. Test results will read as positive, equivocal, or negative and will typically come through in about 5-7 days.

A doctor may run this antibody testing instead of or in conjunction with the Antinuclear Antibody test, the Antiphospholipid Antibodies test, the Anti-dsDNA test, the C-Reactive Protein test (CRP), and a complement test.

Can We Counteract the Anti-Sm Antibody?

A C-type lectin with two identical halves, called CD72, provides hope for effectively battling SLE without causing significant damage to other cells. Research shows that the regulatory protein CD72 actually inhibits the complex formed by anti-sm and RNP antibodies by binding to it.

Trust Only the Best for Important Medical Tests

If your patient presents with signs and symptoms of SLE, put together your panel of lab tests with only the most trusted products and equipment available.

Remember the clinical importance of the anti-Sm antibody when putting together the panel. Contact us for all of your biomedical research kits, genomics, and proteomics and mass spectrometry services.

An Overview of Protein Assay Methods

Posted on

Proteins are one of four macromolecule building blocks that are necessary for any form of life. That’s why being able to analyze proteins with a protein assay is crucial in both research and diagnosis.

Total protein assay kits can be used to determine the total protein concentration of a sample.

To help you choose the right method to measure your protein, read this review of the main protein quantification methods.

Bicinchoninic Acid (BCA) Assay

This colorimetric, two-step protein concentration assay was first developed in 1985. It is relatively young compared to others, such as the Lowry assay.

First, complex the protein by using copper ions. Next, the copper that is now bound with the protein will give off a vibrant purple hue. The shade of purple corresponds to the amount of protein in the sample.

Then, compare the sample to the standard curve to determine the protein.

The BCA assay is a good choice if your protein sample has less than 5% detergents. As this method relies on copper, certain chemicals can interfere with the BCA assay, such as ammonia.

Bradford (Coomassie) Assay

Bradford Assays bind the protein to Coomassie dye in acidic conditions. As a result, the color changes from brown to blue.

When the Coomassie dye binds to the amino acids in the protein, it becomes blue and absorbs at 595 nm. The absorption in your sample can then be compared to a standard curve to determine the protein concentration.

This method is simple, fast, and stable for around 60 minutes. Keep in mind that it only works on proteins larger than 3 kDa. Unlike the BCA, the Bradford is sensitive to certain detergents, such as SDS and Triton X-100.


The Folin-Lowry is a reliable and time-tested protein quantification that dates back to 1951. This has two steps.

Step one: complex copper with nitrogen found in your protein. Step two: the now complex tyrosine and tryptophan will react with the Folin-Ciocalteu phenol reagent to give off a greenish blue shade that absorbs at 650-750 nm.

The great thing about this assay is that the results are stable. So you can estimate how much protein is in your sample by comparing it with a previous standard curve.

The downside of this assay is that it is incompatible with many chemicals and reducing agents.

HPLC (High-Performance Liquid Chromatography)

In a liquid chromatography protein concentration assay, the sample is transported through an absorbent column by a solvent at high pressure.

Each amino acid side chains will react differently. The ones that react least will move down the column more quickly than the side chains that react the most.

As fractionated proteins flow out of the column, each fraction is identified either by UV spectrometry or by mass spectrometry. A dye can be used to make detection easier.

Final Thoughts on Protein Assay

Thanks for reading. We hope you found this list of the various commonly used protein assay quantification methods useful.

Next, check out ProDM, a kit for tryptic digestion monitoring for a successful shotgun proteomics.

ITSIBIO awarded SBA REGION 3 Exporter of the year.

Posted on

… 68 district winners, his nomination was forwarded to their respective regional office – in this case Region 3, which spans Delaware, Philadelphia, Pittsburgh, Maryland, Virginia, Washington, D.C., and West Virginia – where he became the regional winner. The breast cancer screening effort is funded by a grant from the National Institute of Health …

Read full article


Posted on

iTRAQ detects eEF1A1 as a candidate biomarker for Metastatic Progression of Prostate Cancer

ITSI-Biosciences’ latest newsflash publication, “iTRAQ detects eEF1A1 as a candidate biomarker for Metastatic Progression of Prostate Cancer” discusses how PSA is plagued with controversies and is unreliable because of its potential to provide false positive and false negative results. Hence additional biomarkers are needed to replace or compliment PSA to improve detection, diagnosis and prognostication of prostate cancer.

Download full newsflash article


Posted on

ITSI-Biosciences has renewed its Blanket Purchase Agreement (BPA#: HHSN263200900168B) with the NIH

Our products and services are customized for researchers interested in biomarker discovery/validation and protein expression monitoring using human and non-human models. We strive to deliver the best products and quality results to you on a timely basis and within your budget.

We would like to invite you to take advantage of the BPA and have a personal experience with our quality products and customer-focused service. If you order any of our services or products before September 31, 2011, you will receive a 15% special discount (quote “NIH09/31/11”). Please visit our website at: or contact us at: for more information.


Posted on

ITSI-Biosciences is pleased to announce that Concurrent Technologies and Services (CTS) and Biomax Co, LTD have been selected as the 2011 “Most Outstanding Distributor of the Year” and “Best New Distributor of the Year”, respectively.

ITSI-Biosciences distributors’ award recognizes re-sellers of our products for their sales and marketing excellence and for working with ITSI-Biosciences to satisfy the needs of clients.  Under the most outstanding distributor category, distributors were evaluated based on the following:

  1. Percent increase in sales from the previous year.
  2. Total value of products ordered.
  3. Variety of products ordered.
  4. Timely communication of market intelligence and customer feedback to ITSI-Biosciences.


To win the award, a distributor must place first in at least three of the four categories. Concurrent Technologies Nigeria Limited achieved the highest score for total annual product sales, percentage increase in sales for the past 12 months and the variety of products ordered. Funakoshi of Japan, the winner in 2010 and 2011 placed second overall.

Biomax Co Ltd of South Korea won the “Best New Distributor” award.  This award is given to a new distributor who has demonstrated a high level of commitment towards the promotion and selling of our products in their territory. To win the award in the year they were appointed, a distributor must place first in at least two of the following three categories; a) Total value of the products ordered, b) Variety of products ordered and c) Timely communication of market intelligence and customer feedback to ITSI-Biosciences.

In appreciation of their efforts, CTS and Biomax will receive our authentic 2012 Distributor of the Year Award, a certificate and an additional 15% off on all confirmed orders for ITSIPrep Bioanalytical Kits and ITSIPrep Accessories placed with ITSI Biosciences during the month of April 2012.
ITSI-Biosciences congratulates Concurrent Technologies Services and Biomax Co. LTD for this well-deserved award and look forward to continue working with them to increase our market share.

JUNE 22, 2010 Unique New Product Line Launched by ITSI BIOSCIENCES

Posted on

Johnstown, PA ITSI Biosciences is pleased to announce the introduction of a unique line of space-saving laboratory instruments ideal for laboratory and field use. This new line includes the ITSI Vortex Mixer and the ITSI Magnetic Stirrer for use in laboratories and field research.

Both units operate with a small electrical (6V) adapter or batteries (4×1.5v). Each weighing only 200g, they are small, light weight and portable. The ITSI Vortex Mixer and Magnetic Stirrer allow for easy movement around the laboratory with no cable clutter. “You will hardly know they are on the bench until you need to use one. Being battery operated and compact makes these units ideal for field use and for laboratories with limited bench space” says Dr. Richard I. Somiari, President and Chief Scientific Officer.

Dr. Somiari states “We are pleased to announce this addition of portable and space-saving instruments which complements our sample preparation kits and other scientific product line. Both units are cost effective, practical additions for any scientist working in industrial, agricultural, medical, environmental, government or academic research.”

The Vortex Mixer and Magnetic Stirrer can be purchased online at or by calling ITSI Biosciences (814)262-7331.

MAY 20, 2010

Posted on

ITSI-Biosciences ( today announced that Cheryl Napier, M.S is the lucky winner of the Acer Net Book that was displayed at ITSI- Biosciences booth during the 2010 American Association for Cancer Research Annual Meeting in Washington D.C from April 17 – 21. Mr. James White, Economic Development Coordinator for the City of Johnstown, PA drew the lucky entry from over 100 entries.


Posted on

Johnstown, Pennsylvania, USA ITSI Bioscience a leading global proteomics, genomics and bioreagent solutions company located in Johnstown, PA has awarded Funakoshi Co., Ltd of Tokyo, Japan their prestigious Distributor of the Year Award for 2009.

Criteria for the award included new sales volume, market penetration and customer satisfaction. This year’s award winner, Funakoshi Co., Ltd has contributed to the growth of Johnstown based Integrated Technologies and Services International (ITSI Biosciences) as an important life science company supplying innovative products to the scientific communities in Japan.To win the award, Funakoshi placed at the top of all three criteria ahead of distribution partners located throughout Africa, Asia, North America, South America and the Middle East.

Dr. Richard Somiari, PhD, President & CEO, indicates “The Distributor of the year award is a way for us to say thank you to a company who has worked with ITSI as an invaluable team member and contributing to our overall success.” “We are honored to work with an organization like the Funakoshi Company to achieve our corporate goals. The international market place is a very competitive environment and successful partnerships abroad are essential to our continued international growth trend.”

“I don’t know if we are worthy of such a great honor, but we are grateful. It will encourage us to make more efforts to expand the market share of ITSI Biosciences in Japan,”  says Shinya Ichikawa, Marketing Specialist, International Business Department at Funakoshi Company.

In appreciation of their efforts, Funakoshi Co. will receive a 2009 Distributor of the Year award, certificate and an additional 10% off all orders placed with ITSI Biosciences during the month of February 2010.

DECEMBER 04, 2009

Posted on

ITSI Biosciences is pleased to announce the addition of Richard Cosgrove as Vice President of Operations and Business Development to the organization.

Mr. Cosgrove will be directly responsible for all aspects of business development and marketing to help ITSI Biosciences expand its domestic and international market share in academic, corporate and government sectors, as well as managing operations of the company™ business units.

According to Richard Somiari, PhD, President and CEO, “We are extremely pleased to have Rick join us at this important time in the growth of the company. Our future in the national and international market place is very bright, and Mr. Cosgrove will focus on consolidating existing markets and working to open up new business opportunities in government, the defense industry and pharmaceutical companies”.

Mr. Cosgrove comes with extensive marketing and management experience in the healthcare industry. He holds a Master’s degree in Liberal Arts/International Studies from Lock Haven University, Advanced Graduate Studies International Business Management certificate from Point Park University and a Bachelor of Science degree, in Marketing from Indiana University of Pennsylvania. Rick has previously held positions at HealthSouth Corporation and University of Pittsburgh Medical Centers and is Adjunct Professor of Marketing/Management at the University of Pittsburgh at Johnstown. He is a member of the Board of Directors of the Greater Johnstown/Cambria County Chamber of Commerce, where he is the chairman, Government Action Committee. Mr. Cosgrove is a member of the I.U.P.  Eberly College of Business and Technology’s business advisory board, Johnstown Symphony Orchestra’s board of advisors and is chairman of the American Heart Association Cambria/Somerset Heart Ball 2010.

DECEMBER 4, 2008

Posted on

New lab, NCI funding provide ITSI-Biosciences with broader base for new breast-cancer detection test is featured in Keystone Edge.

NOVEMBER 24, 2005

Posted on

ITSI-Biosciences and President and CSO Richard Somiari Ph.D. is prominently featured in the 11/24/05 issue of Nature.


Posted on

Proteomics on Formalin-Fixed Parafin-Embedded Tissues

ITSI-Biosciences’ latest newsflash publication, “Proteomics on Formalin-Fixed Parafin-Embedded Tissues” discusses how Formalin-Fixed Paraffin Embedded Tissues (FFPE) are commonly used in healthcare to preserve tissues from a wide variety and different stages of a disease. However formalin cross links the protein and poses a great obstacle for downstream proteomics application.

Download full newsflash article


Posted on

ITSI-Biosciences’ latest application note, “ITSIPrep Kits for Two Dimensional Difference in Gel Electrophoresis” discusses how to:
1) Achieve great results in 2D-DIGE experiments.
2) Streamline and standardize the up-stream sample preparation steps including protein isolation, fractionation, precipitation and depletion.
3) Avoid the use of poor quality reagents and inadequate protocols which results to poor quality data and waste of time and money.

Download full application note


Posted on

ITSI-Biosciences wins Small Business Exporter of the Year, and is being awarded by The Saint Francis University Small Business Center, Friday June 17th, 2011. This award recognizes the outstanding achievements of the Southern Alleghenies Region’s most successful entrepreneurs and small business advocates.


Posted on

ITSI-Biosciences featured in Commercial News USA, the official export magazine of the U.S. Department of Commerce.
Commercial News USA is the premiere export promotion program for promoting U.S. goods and services in more than 178 countries around the world. The catalog-style magazine reaches more than a quarter million buyers and distributors worldwide. The magazine is direct-mailed to subscribers around the world, and also is distributed at U.S. embassies and consulates, as well as at major trade shows.

In addition to us being included in the print edition, we receive a free listing, email and Web links in both the pdf and html versions of the magazine on their Web site.

Click here to view advert


Posted on

Funakoshi Co., Ltd of Tokyo a leading distributor of bioreagents in Japan has been named the ITSI-Biosciences 2010 “Distributor of the Year”. This is the second time that Funakoshi is wining this distinguished and highly competitive award. Funakoshi Co., Ltd has continued to contribute to the growth of ITSI-Biosciences products in Japan.

Criteria for selecting the award winner included a) overall number of items ordered in 2010, b) frequency of order, c) the different types of products ordered d) communication of feedback from customers to ITSI-Biosciences. To win the award, a distributor must place first in at least three of the four categories. Funakoshi placed first in three categories and second in one category, outperforming other international distributors.

According to Dr Richard I. Somiari, President/CSO, “this unique partnership leverages Funakoshi and ITSI-Biosciences strengths to bring innovative life science products to scientists in Japan at a competitive price”.

“It is a great honor to receive this award. We really hope that ITSI-BIOSCIENCES will dramatically boost their market forces in 2011. Of course, we will also exert our utmost efforts to establish their market in our sales territory as before.” says Shinya Ichikawa, Marketing Specialist, and International Business Department at Funakoshi Company.

In appreciation of their efforts, Funakoshi Co. will receive an authentic 2010 Distributor of the Year award, a certificate and an additional 10% off on all orders placed with ITSI Biosciences during the month of March 2011.

Click here to view Award

December 7, 2010 Christopher P. Koop, from Congressman Mark Critz office visited ITSI-Biosciences

Posted on

Mr. Koop toured the new state-of-the-art facility to learn more about ITSI’s business and operations in Johnstown. As Congressman Critz’s field representative Mr. Koop is helping ITSI-Biosciences establish strategic relationships, expand operations and take advantage of the unique opportunities for life science and biotechnology companies in the Johnstown area and 12th congressional district.

June 30, 2010 Visiting PhD Research Fellows commence Breast Cancer and Sickle Cell Research at ITSI-Biosciences

Posted on

Two PhD Research Fellows from Olabisi Onabanjo University, Nigeria, arrived in Johnstown, PA today to commence research programing at ITSI – Biosciences. The two students will be in the United States for 6 months researching on Sickle Cells and Breast Cancer.

Picture Legend from the left: Mutu A. Alabi (Phd Research Fellow in Breast Cancer), Teresa Dugan (Consultant/Training Specialist) and Olaleken H. Oyefuga (Phd,Research Fellow in Sickle Cell)

July 28, 2010 President and Chief Executive Officer of Protein Discovery visits ITSI-biosciences

Posted on

Chuck Witkowski founder of Protein Discovery visits ITSI-Biosciences facility today. Mr Witkowski is is a visionary leader with demonstrated expertise in building successful medical and life science companies from inception.

Picture Legend from the left: Dr. Richard Somiari (President & Chief Scientific Officer of ITSI-Biosciences), Chuck Witkowski (President & Chief Scientific Officer)

June 21, 2010 ITSI-Biosciences host summer interns

Posted on

“ITSI – Biosciences host summer interns”. Five students will be at ITSI – Biosciences for 7 weeks as interns. Three are high school graduates; Nicole Danchanko that will be going to Penn Highlands Community College in the fall to study Biology, Richard Somiari Jr going to Lehigh University also in the fall to study Computer Engineering and Jamie Lepsch going to Robert Morris University to also study Computer Engineering. Two are college students; Zach Evans a sophomore from the George Washington University majoring in International Affairs, Jasmine LaRue majoring in Biology at Mount Aloysius College.

March 01, 2010 DHQ KMS personnel commence training in San Antonio

Posted on

ITSI, LLC today announced the commencement of the second phase of the training for the visiting Nigerian Defence Headquarters (DHQ) Knowledge Management System (KMS) project personnel in San Antonio, TX.

This phase of the training is provided by BIF Technologies of San Antonio, TX. BIF is ITSI’s technical partner for implementation and management of the DHQ-KMS program.

March 1, 2010 Nigerian Armed Forces DNA Bank and Analysis personnel visit Cybergenetics Corporation, Pittsburgh, PA.

Posted on

ITSIBIO today announced that ITSI Biosciences and Cybergenetics ( are collaborating to train the Nigerian Armed Forces DNA Bank (AFDB) personnel, and implement modern methods for forensic DNA analysis and interpretation in Nigeria. As part of this strategic relationship 6 AFDB personnel undergoing training at ITSI Biosciences since September 2009 have visited and trained at the CyberGenetics facility in Pittsburgh, PA from December 16th 17th, 2009. Cybergenetics is a leader in producing unique computational approaches for automating and simplifying forensic DNA data processing, analysis and interpretation. The Cybergenetics TrueAllele® system enables rapid and automated forensic DNA data analysis and interpretation.

February 17, 2010 2B Scientific, United Kingdom appointed distributor for Europe.

Posted on

ITSIBIO has signed a representation and distribution agreement with 2B Scientific Limited, Upper Heyford, United Kingdom. 2B Scientific (, a specialist reagents distribution company to the UK Life Science Markets will serve as a springboard for European markets. This appointment gives 2B Scientific the rights to promote, market, distribute and sell ITSIBIO products in Europe. Clients in this territory can now place orders for all ITSIBIO products through 2B Scientific.

February 4, 2010 Nigerian Defense Headquarters, ITSI – Biosciences representative visit Nigerian Armed Forces DNA Bank (AFDB) in Abuja, Nigeria.

Posted on

ITSIBIO today announced that Nigerian Defence Headquarters Research and Development leaders, Major Gen BT Obasa (Chief, Research & Development) and Air Vice Marshall A Badeh (Director of Research) and Dr Richard I. Somiari (President & CSO) ITSI – Biosciences, LLC toured the Nigerian Armed Forces DNA Bank at Mogadishu Barracks, in Abuja, Nigeria. ITSI Biosciences is helping the Nigerian DHQ develop a Genvault technology based DNA bank capable of banking DNA from 100,000 military personnel. A total of 6 AFDB personnel are currently undergoing DNA banking and analysis training at ITSI Biosciences, Johnstown PA. As part of the training, the AFDB personnel have also spent time at Indiana University of Pennsylvania, Indiana, PA, Cybergenetics, Pittsburgh, US Armed Forces DNA Identification Laboratory (AFDIL), Rockville, MD. The visit was to assess the readiness of the facility to receive the trainees who will return to Nigeria in April 2010.

Picture Legend: Nigerian DHQ Research & Development leaders and ITSI representative visit the Armed Forces DNA bank in Abuja, Nigeria. From right: Maj Gen BT Obasa (Chief Research & Development, DHQ), Richard I. Somiari, PhD (President & CSO, ITSI Biosciences) and AVM Badeh (Director of Research, DHQ)

January 20, 2010 Nigerian Armed Forces DNA Bank (AFDB) personnel visit US Department of Defense AFDIL and AFRSSIR.

Posted on

ITSIBIO today announced that 6 Nigerian AFDB personnel visited the United States Department of Defense (DoD) Armed Forces DNA Identification Laboratory (AFDIL; in Rockville, MD and Armed Forces Repository of Specimen Samples for the Identification of Remains (AFRSSIR; in Gaithersburg, MD. The visit is part of the comprehensive forensics DNA banking and analysis training organized by ITSI Biosciences, Johnstown, PA for the Nigerian Armed Forces DNA Bank and Analysis personnel. The visit allowed the AFDB personnel to tour both facilities and observe first-hand how DNA is collected, stored at room-temperature and analyzed by the US military in support of military missions.

December 9, 2009 US Armed Forces DNA Identification Laboratory (AFDIL) Education team arrive Johnstown, PA to train Nigerian Armed Forces DNA Bank personnel.

Posted on

ITSIBIO today announced that instructors from AFDIL based in Rockville, MD have arrived Johnstown, PA to help train Nigerian Armed Forces DNA Bank (AFDB) personnel. The AFDIL team led by Michael D. Coble, PhD (Research Section Chief) arrived in Johnstown, PA on the 8th of December 2009. According to Richard Somiari, PhD, President of ITSI, LLC “the training will focus on military applications and cover; 1) Forensic DNA Analysis 2) Short Tandem Repeat (STR) Data Analysis and Interpretation 3) Population Genetics and Statistics and 4) Non-STR DNA Markers. ITSI LLC (; is helping AFDB setup a state-of-the-art DNA banking and analysis center in Abuja, Nigeria that will be capable of banking DNA from up to 100,000 military personnel. The involvement of AFDIL in the training program is critically important and key to achieving the overall project goals added Dr Somiari.

December 7, 2009 Nigerian Defence Headquarters personnel commence computer science and Knowledge Management System training in Johnstown, PA.

Posted on

ITSI, LLC today announced the arrival of 6 Nigerian Defence Headquarters (DHQ) personnel in Johnstown, PA to commence five months computer science and knowledge management system training. According to Richard I Somiari, President and CEO of ITSI, the training is part of the MOU between DHQ and ITSI concerned with the design, construction and setting up of a prototype knowledge management system for DHQ. Several organizations including Pennsylvania Highlands College, Johnstown PA; BIF Technologies, San Antonio, TX and ThreatGuard, San Antonio, TX have teamed up with ITSI, LLC to provide the training.

December 04, 2009 ITSI Biosciences is plea sed to announce the addition of Richard Cosgrove as Vice President of Operations and Business Development to the organization.

Posted on

Mr. Cosgrove will be directly responsible for all aspects of business development and marketing to help ITSI Biosciences expand its domestic and international market share in academic, corporate and government sectors, as well as managing operations of the company’s business units. According to Richard Somiari, PhD, President and CEO, “We are extremely pleased to have Rick join us at this important time in the growth of the company. Our future in the national and international market place is very bright, and Mr. Cosgrove will focus on consolidating existing markets and working to open up new business opportunities in government, the defense industry and pharmaceutical companies”.

Mr. Cosgrove comes with extensive marketing and management experience in the healthcare industry. He holds a Master’s degree in Liberal Arts/International Studies from Lock Haven University, Advanced Graduate Studies International Business Management certificate from Point Park University and a Bachelor of Science degree, in Marketing from Indiana University of Pennsylvania. Rick has previously held positions at HealthSouth Corporation and University of Pittsburgh Medical Centers and is Adjunct Professor of Marketing/Management at the University of Pittsburgh at Johnstown. He is a member of the Board of Directors of the Greater Johnstown/Cambria County Chamber of Commerce, where he is the chairman, Government Action Committee. Mr. Cosgrove is a member of the I.U.P.  Eberly College of Business and Technology’s business advisory board, Johnstown Symphony Orchestra’s board of advisors and is chairman of the American Heart Association Cambria/Somerset Heart Ball 2010.

September 02, 2009 Armed Forces DNA Bank (AFDB) personnel commence DNA banking and analysis training at ITSI-Biosciences

Posted on

Six members of the Nigerian Armed Forces DNA Bank (AFDB) representing the Army, Navy and Air force, arrived in Johnstown, PA today to commence DNA banking and analysis training at ITSI Biosciences. The team will be in the United States for 8 months learning new DNA banking and analysis methods. According to Richard Somiari, PhD, President of ITSI Biosciences, the training is part of the strategic relationship between ITSI Biosciences and the Nigerian Defense Headquarters concerned with the establishment of a state-of-the art DNA bank and analysis center for the Nigerian military. When fully implemented, the Nigerian Defence Headquarters DNA banking initiative which is the first military large-scale DNA banking initiative of its kind in Africa will have capacity to bank DNA from up to 100,000 military personnel.

AFDB Personnel: Abiodun Ebenezer Adebiyi(Lt. Col, Army), Chidi Innocent Onuoha(L/Cpl, Army), Olarewaju Anthony Ogundele(PO Navy), Anthony Friday Akpan(FS, Air Force), Ikwoegbu Mba Igbe(Sqn. Ldr, Air Force), Livinus Ukachukwu(Lt. Cdr. Navy).

February 1, 2009 ITSI Biosciences moves into new larger facility with ISO Certified Clean Room

Posted on

ITSIBIO today announced its movement into its new and larger facility. The new state-of-the-art facility in the Kernville district of Johnstown, PA is a green building with a silver status. The new facility includes more office space, expanded research laboratory and kit manufacturing space, and an ISO Certified Class 1000 clean room. This move is another step in our company’s growth strategy states Richard Somiari, PhD, founder and president of ITSI Biosciences. ITSIBio continues to be successful in delivering innovative services, developing easy-to-use sample preparation kits and securing competitive grants for cancer biomarker development.

January 19, 2009 ITSI Biosciences launches packaging for ITSIPrep products

Posted on

ITSIBIO today announced the introduction of a new and improved packaging for its distinctive line of ITSIPrep products. The new colorful and distinctive packaging is a marketing tool that reflects the key attributes of ITSIPrep line of products. This new packaging replaces the plain white package that the company has been using for three years. According to Richard Somiari, PhD, President of ITSI Biosciences this new package will distinguish ITSI Biosciences products, and provide better protection to the contents during shipment of our innovative products to customers in the USA and around the world.

January 5, 2009 ITSI Biosciences is a subrecipient of a 5 year $2 million RO1 grant to study prostate cancer

Posted on

ITSI Bioscience has announced that it is a Subrecipient of a $2 million 5 year RO1 grant awarded to Dr Karam El-Bayoumy of Penn State University Cancer Center. This NCI subaward will fund proteomics research at ITSI Biosciences, focusing on the identification of biomarkers of prostate cancer risk. Specifically, ITSIBio will utilize high performance 2D-DIGE MS and nano-LC/MS/MS to identify and characterize redox-sensitive proteins, and global proteomic patterns associated with the form and dose of selenium administered to human subjects, and those associated with the age of the subjects.

December, 2008 New lab, NCI funding provide ITSI-Biosciences with broader base for new breast-cancer detection test

Posted on

Dr. Richard Somiari, the founder of ITSI Biosciences, a life-science enterprise in the Johnstown region, is preparing to move his company into its new headquarters by year’s end–just in time to expand field investigations of a blood-sampling method that promises to increase the speed and lower the cost for early detection of breast cancer. That work received a $100,000 Small Business Innovation Phase I Research Grant from the National Cancer Institute in July.

After what appears to be a long search for the right space, ITSI-Bio’s third move promises to be a charm for a four-year-old business that represents a bellwether for the efforts to attract a cluster of life-science enterprises to the Johnstown region.

“We’ve been carrying on our work in about 1,500 square feet and need the flexibility of a custom-built laboratory,” Somiari says from his present location in the Kernville neighborhood of Johnstown, Cambria County. The new location will place the company, which provides analytical services and sampling kits for bioscience laboratories worldwide, in a 10,000-square-foot facility nearer the heart of Johnstown.

ITSI-Bio will initially occupy half of that area, where it will be able to broaden its basic analytical services to bioscience researchers. In addition to analytics, the company also offers kits for isolating, handling, testing, and monitoring proteins, as well as reagents used to tag biochemical reactions. “That business has kept the doors open while we have pursued our method for early breast cancer detection,” Somiari says.

The detection protocol draws upon about 20 biological markers, some of which have been clearly associated with breast cancer and others that Somiari has identified for the blood-sample analysis that he hopes to demonstrate as a faster and more precise testing method.

While that goal has directed his passion as a scientist, he has been closely identified with the future of biotech in Johnstown. He came to the region from the University of Maryland in 2000 to start the Windber Research Institute, the region’s first biomedical research institute. Only later did he form his own company to develop a commercial product–deciding, as he says, that it would be a good idea to practice the entrepreneurship that he had been preaching.

The NCI grant will finance expansion of the breast-cancer-test sampling and independent validations of results through UPMC McGee Hospital, Indiana Regional Medical Center, and the Department of Biomedical Informatics at the University of Pittsburgh. It will also support the development of algorithms to analyze results, as well as development of a Phase II NCI grant for up to $1 million.

Source: ITSI-Biosciences, Richard I. Somiari
Writer: Joseph Plummer
Original Article:

November, 2008 ITSI Biosciences receives $100,000 Phase 1 SBIR Grant from NCI for breast cancer research

Posted on

ITSI – Bioscience has announced the receipt of a $100,000 Small Business Innovation Research (SBIR) Phase 1 grant from the National Cancer Institute. The grant will support ITSIBIO’s ongoing blood-based breast cancer detection biomarker program that promises to provide an alternate method for earlier detection and accurate classification of breast malignancies.

At the moment mammography is the most widely used screening tool for breast disease detection and diagnosis. Unfortunately, mammography can miss some breast cancers, especially in young women with dense breast, it cannot unequivocally distinguish between benign and malignant lesions and it can not be performed remotely. If successfully validated, the proposed blood test would be beneficial to all women who show abnormalities in their mammograms and require further testing. Most of these women currently endure biopsies, up to 80% of which are probably unnecessary, as no cancer is found.

According to Richard Somiari, PhD principal investigator for the study titled “development of a blood test for breast cancer detection” the grant will support a) the collection of blood samples from informed and consented female donors, b) multiplexed assay development using a suspended array technology and c) development of a prototype breast disease classification model. While there are other efforts around the world to develop biomarker based tests for breast disease detection and classification the “ITSIBIO blood test is unique in its approach, because, it could allow accurate classification of breast disease and employ a reliable and sensitive blood RNA detection method that does not require RNA isolation and polymerase chain reaction” added Dr Somiari.

March, 2008 ITSIBIO signs distribution agreement with Precision Technologies PTE Ltd, Singapore

Posted on

ITSIBIO has signed a representation and distribution agreement with Precision Technologies PTE Ltd, Singapore ( This agreement, which is effective March 17th 2008, gives Precision Technologies exclusive rights to promote, market and distribute ITSIBIO products in Singapore, Malaysia and Indonesia. Clients in this territory can now place orders for all ITSIBIO products through Precision Technologies. Orders can be place by Phone: +6273-4573, Fax 6273-8898 or E-mail:

January, 2008 NCI extends Blanket Purchase Agreement with ITSIBIO to 2010.

Posted on

NCI has extended the Blanket Purchase Agreement (BPA), originally signed with ITSIBIO in 2005, until 2010. This highly successful BPA which is managed by SAIC-Frederick, allows all NCI researchers to purchase genomics, proteomics and mass spectrometry services, and consumables from ITSIBIO at a pre-negotiated price. The BPA streamlines the process of outsourcing analytical support and locks-in lower prices for all investigators by leveraging economy of scale. Because the BPA contains legally binding confidentiality and non-disclosure clauses, researchers can fully discuss their experiments with the confidence that their ideas and scientific results will be safe and protected to the full extent required by law.

ITSIBIO receives Human Subject Research and ICH-GCP Certification.

ITSIBIO has received the Human Subject Research and International Conference on Harmonization (ICH) Good Clinical Practice (GCP) certifications. ICH-GCP brings together the regulatory authorities of Europe, Japan and the United States and experts from the pharmaceutical industry to ensure protection of human subjects and allow a more economical use of human, animal and material resources, and the elimination of unnecessary delay in the global development and availability of new medicines whilst maintaining safeguards on quality, safety and efficacy, and regulatory obligations to protect public health. ICH-GCP facilitates the dissemination and communication of information on harmonized guidelines, and the use such information/guidelines to encourage the implementation and integration of common standards. This significant milestone means that ITSIBIO’s staffs have a working knowledge of the principles and ethics associated with good clinical practices, understands Good Clinical Practices dictated by FDA ( and the ICH Requirements for Sponsors, Monitors, and Investigators. ITSIBIO affirms that all projects performed at ITSIBIO will strictly adhere to Use of Human Subject Research and ICH-GCP guidelines to allow the submission of the data for regulatory approval. Both certifications were received through the Collaborative Institution Training Initiative (CITI,

ITSIBIO signs agreement with BioConsult, Jerusalem, Israel.

ITSIBIO has appointed BioConsult, Jerusalem, Israel ( ) as the exclusive distributor and representative in Israel. The agreement signed December 2007, gives BioConsult exclusive rights to promote, market and distribute all ITSIBIO products and services in Israel. Clients in Israel can now place orders through BioConsult via phone or by visiting their website.

ITSIBIO signs collaboration agreement with University of the Western Cape, Bellville, South Africa.

ITSIBIO has signed a collaboration agreement with the University of Western Cape Proteomics Research Group. Under the agreement, signed December 2007, both parties will collaborate in the areas of proteomics and sample preparation reagent development.

ITSI-Biosciences signs collaboration and distribution agreement with Innovative Biotech Ltd, Keffi Nigeria.

ITSIBIO enters the Nigerian market by signing a collaboration agreement with Innovative Biotech Limited (IBL), Keffi, Nigeria ( Under the agreement signed November 2007, ITSIBIO and IBL will collaborate to develop diagnostic assays for infectious diseases of national interest to Nigeria and the West African region. A distribution agreement signed in December 2007 during the visit of Simon Agwale, PhD, IBL CEO, to ITSIBIO head office in Johnstown, PA also gives IBL the exclusive rights to market, distribute and support ITSIBIO, Panomics Inc. and GenVault Inc products and services in Nigeria. All orders in the Nigerian market should now be placed through IBL Nigeria.

Bin Ali Medical Supplies appointed distributor for the Middle East market.

ITSIBIO announced today the appointment of Bin Ali Medical Supplies (BAMS), Abu Dhabi, United Arab Emirates ( as the exclusive distributor and representative in the Middle East. The agreement signed in October 2007, allows BAMS to promote, market and distribute all ITSIBIO’s products and services in the Middle East. Clients in Middle East can now place orders through BAMS.

May, 2007

Posted on

ITSIBIO enters the Indian market by signing an exclusive agreement with SR Biosytem of New Delhi. Under the agreement signed May 2007, SR Biosytem will promote, market and distribute all ITSIBIO’s line of products, accessories and services in India. All orders in the Indian sub-continent should now be placed through SR Biosystem.

April, 2007

Posted on

ITSI signs an exclusive agreement with Separation Scientific (SA) (PTY) Ltd, of South Africa. Under the agreement that is effective from April 2007, Separation Scientific will promote, market and distribute all ITSIBIO’s products and services in the South Africa and other neighboring countries. Clients in Southern Africa should now place orders through Separation Scientific (SA) (PTY).

December 5 , 2006

Posted on

Pennsylvania Governor Rendell announces the investment of $500,000 to enable ITSI to expand its research facility and operations.

September 28, 2006

Posted on

ITSI representative invited to speak at the South African Bio2Biz International Biotechnology Conference, Durban, South Africa. A paper titled From Genomics and Proteomics to Bio-Business presented.

September 12, 2006

Posted on

ITSI representative invited to speak at the International Symposium on Inflammation: An Underlying Factor in Several Diseases, Ibadan, Nigeria. A paper titled Role of Proteomics in Inflammation Research presented as a keynote lecture.

September 7, 2006

Posted on

ITSI signs a collaboration agreement with the Advancement Center, College of Medicine, University of Nigeria. Under this agreement ITSI will work with the College of Medicine to develop integrated biomedical research programs and a biorepository to support cancer and complex disease research.

August 15, 2006

Posted on

ITSI signs an exclusive Representative and Distributorship agreement with InterSciences Inc., Markham Ontario, Canada. Under this agreement, InterSciences will promote, market and distribute the ITSIPREP™ line of products and easy-to-use kits for protein isolation, protein fractionation, protein precipitation, albumin removal and protein assay in Canada.

June 2006

Posted on

Dr Munir O. Azeez (Col.) of the Nigerian Army Medical Corps (NAMC) joins ITSI for six months advanced biomedical research training, as part of the training and collaboration agreement between ITSI and NAMC. Dr Azeez is interested in applying advanced proteomic technologies to study tissue from patients diagnosed with temporo-mandibular joint disease.

May 2006

Posted on

ITSI signs an exclusive distribution agreement with Funakoshi Japan. Funakoshi will promote, market and sell the ITSIPREP™ line of products in Japan.

April 2006

Posted on

ITSI signs a non-exclusive Sales Representative Agreement with Grace Scientific LLC, Clarksburg, MD, USA. Grace Scientific will promote, market and sell ITSI’s services, especially 2D-DIGE and 2D-Image analysis services and all the ITSIPREP™ line of products in the Maryland, Washington DC and Virginia area.

March 2006

Posted on

ITSI signs an exclusive Representative and Distributorship agreement with Rainbow Biotechnology Co., Taipei, Taiwan. Rainbow will promote, market and distribute the ITSIPREP™ line of products including optimized and easy-to-use kits for protein isolation, protein fractionation, protein precipitation, albumin removal and protein assay.

February 1 2006

Posted on

Itsi-Biosciences has moved to a new, larger location. Images of our new facility and additional information will available online shortly. Please see the Contact page for our new address.

January 2006

Posted on

ITSI appoints Syngenic Biotecnologia Ltda., Sao Paolo Brazil as the exclusive distributor of the ITSIPREP™ line of products in Brazil. The agreement, which was signed in October 2005, covers all the optimized and easy-to-use kits for protein isolation, protein fractionation, protein precipitation, albumin removal and protein assay.

November 24 2005

Posted on

ITSI-Biosciences President and CSO Richard Somiari Ph.D. is prominently featured in the 11/24/05 issue of Nature.

September 14 2005

Posted on

Itsibio represented at GE Healthcare 2D-DIGE Users Group meeting in Nashville, TN. Presents paper “Application of 2D-DIGE technology for the analysis of clinical specimens.”

September 9 2005

Posted on

Robin L. Fisher, Director Asia & Europe, International Business Development, PA DCED, Mr. Donald M. Bonk, International Trade Manager, SAPDC and Ms. Sushama A Kanetkar, Director, PA Trade Representative Office in India, and Supriya, Trade & Investment Advisor, PA Trade Office India visit Itsibio corporate headquarters/laboratory to discuss export opportunities and entry into the Indian market.

September 8 2005

Posted on

ITSIBIO invited to exhibit products at the Cambria County Chamber of Commerce Small Business Expo in Ebensburg, PA

August 12 2005

Posted on

Itsibio, Applied Isotope Technologies and MountainTop Technologies sign agreement to explore business opportunities of mutual interest involving proteomics.

August 5 2005

Posted on

Itsibio signs a distribution agreement with Biomedical Marketing Associates. BMA will market and distribute ITSI’s proteomics products including protein isolation, fractionation and assay kits in 5 states: Michigan, Ohio, Kentucky, West Virginia and Western Pennsylvania.

July 22 2005

Posted on

David H. Fyock, Chairman/CEO, MountainTop Technologies Inc., Matt Pamuku, President, Applied Isotope Technologies, Inc., Prof. HM (Skip) Kingston, PhD, Duquesne University and Dr. Steve Ayanruoh, MD, Chairman, Ruskat Medical Equipment Corp and Applied Medical Technologies, visit Itsibio to discuss collaboration and teaming agreement.

July 15 2005

Posted on

Stephen C. Metzger, Director Business Lending Services of Southern Allegheny Planning & Development Commission, Altoona, PA visits itsibio to discuss funding opportunities and support. SAPDC is supporting itsibio’s effort to export bioanalytical reagents produced in Johnstown, PA to Mexico and Brazil, and to access funds through the Small Business First loan program.

July 11 2005

Posted on

Prof. Francis A.A. Adeniyi, Dean, Faculty of Basic Medical Sciences and Ag. Director, Institute for Advanced Medical Research & Training (IMRAT), University of Ibadan, Ibadan, Nigeria visits itsi-Biosciences to discuss collaboration in the areas of biomedical research, bioanalytical support, tissue banking and data center development.

July 8 2005

Posted on

Itsibio acquires ProteinChip System Series 4000 Enterprise Edition Surface Enhanced Laser Desorbed Ionization – Time of Flight (SELDI-Tof) fromCiphergen Biosystems as part of an emerging collaboration between Itsibio and Ciphergen. The system, which is the best for analysis of serum, plasma, urine and saliva will support internal biomarker discovery programs and will enable Itsibio to provide contract biomarker discovery services to academia and pharmaceutical industries.

July 7 2005

Posted on

Itsibio representatives visit NCI-Frederick, Frederick MD. Present seminar “Integrating emerging high throughput technologies for complex disease research”

July 6 2005

Posted on

Itsibio representatives visit GE Healthcare, Piscataway NJ. Meet with Andy Bertera, Head of Marketing, Gene & Protein Discovery.

June 20 2005

Posted on

Itsibio attends Bio 2005, meets with potential partners from around the world.

April 19 2005

Posted on

Mr. Joseph Burke, Director, Americas, So. Africa, Mid. East & Oceania Grp., PA DCED, Mr. Donald M. Bonk, International Trade Manager, SAPDC and Ms. Silvia Alonso, Senior Trade Specialist for PA in Mexico visit itsibio corporate headquarters/laboratory.

April 13 2005

Posted on

Itsibio attends American Society of Breast Disease (ASBD) annual meeting (April 13-16 2005) in Las Vegas, NV. Itsibio representative serves on the Outreach and special populations committee and is a member of the Expert working group on risk assessment of the ASBD.

March 22 2005

Posted on

Itsibio representative visits University of Nigeria, Nsukka, Nigeria and presents seminar “Emerging high throughput technologies and complex disease research” Meets with Director, University Resource Foundation, Provost, University of Nigeria Teaching Hospital, Chief Medical Director, University of Nigeria Teaching Hospital and other senior faculty and university administrators to discuss partnership between both organizations.

March 19 2005

Posted on

Itsibio representative visits the Institute of Advanced Medical Research & Training, University of Ibadan, Nigeria and University College Hospital, Ibadan, Nigeria. Presents a seminar &;Advances in High throughput Biomedical Research & Personalized Medicine. Meets with Prof E. Bababunmi, Prof. Francis AA Adeniyi and Prof. Idowu Yinka and other senior faculty members to discuss partnership between both organizations.

March 18 2005

Posted on

Itsibio representative attends the launching of the Nigerian Army Medical Corps/School (NAMC/S) rollback malaria program. Meets with the Honorable Minister of State for defense Dr. Roland Oritsejafor, Lt. Gen ML Agwai (Chief of Army Staff) and Maj. Gen B. E. Abang (Commandant NAMC/S).

March 4 2005

Posted on

Itsibio representative visits and presents a seminar; Application of emerging high throughput technologies in complex disease research; at the College of Pharmacy, University of Houston, TX. Meets with Dean Sunny Ohia and senior faculty.

February 02 2005

Posted on

Itsibio and Asterand Inc. sign collaboration agreement.

January 3 2005

Posted on

Itsibio formally opens ultra-modern laboratory for genomics and proteomics research and provision of bioanalytical services.