ITSIPrep Protein Digestion Monitoring (ProDM) Kit is the first kit to allow fast and accurate monitoring of the protein digestion step without performing gel electrophoresis. ProDM kit will allow researchers easily and quickly test the trypsinized sample to accurately determine the extent of digestion. All enzymes can be monitored including trypsin, chymotrypsin.
ITSI-BIO Buccal DNA Collection Kit (K-0022) is an easy-to-use kit validated specifically for the collection of buccal DNA sample. It is a reliable and inexpensive alternative to the more expensive kits. It can be used to collect cheek cells from which DNA will be isolated.
ITSI-Biosciences distinctive Western Blotting Kit (W-Blot™ [K-0045]) is a ready-to-probe western blot of human, animal, or plant proteins on a nitrocellulose membrane (Figure 1, Figure 2). Each blot contains 20ug of total protein per lane. W-Blot™ can be re-probed multiple times and can be customized with any required tissue. The PVDF membrane is available upon request.
ITSIPrep Trypsin Digestion Monitoring (TrypDM) Kit is the first kit to allow fast and accurate monitoring of the level of protein digested when trypsin or any other enzyme is used. No gel electrophoresis is required to confirm that the protein is digested. The TrypDM kit will enable researchers to easily and quickly test the trypsinized sample to accurately determine the extent of digestion.
This Proteomics Grade Plasma Tube (PGPT) is specifically developed for collection of plasma specimens. PGPT contains Gel, Lithium Heparin and five-protease inhibitors with specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. PGPT reagents instantly inactivate proteases thereby preventing changes in the proteome attributable to ex-vivo protease activity.
This Proteomics Grade Serum Tube (PGST) is developed specifically for collection of serum specimens. The tube contains five-protease inhibitors with specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. All samples can be analyzed by mass spectrometry, SDS-PAGE, 2D-DIGE and Western blotting.
The Quanti-Protein Assay Reagent – Nano (nQ-PAR) is an optimized ready-to-use reagent for quantitation of total proteins using the NanoDrop ND1000 spectrophotometer. nQ-PAR can be used to quantify proteins isolated from microorganisms, cell lines, whole tissue, blood, serum and plasma. nQ-PAR is compatible with many procedures, can be stored at room temperature, and tolerates many common laboratory buffers. Available in 50 or 100 assays.
The Protein Standard Curve Reagent kit provides eight concentrations (25ug/mL – 2000ug/mL) of Bovine Serum Albumin in a ready-to-use and room temperature stable format. This product improves reproducibility and reduces the time required for Bradford assay-based standard curve preparation, and hence accuracy and ease of protein quantitation. Available in 50 or 100 assays.
The Quanti-Protein Assay Reagent (Q-PAR) is an optimized ready-to-use reagent for quantitation of total protein. Q-PAR can be used to quantify proteins isolated from microorganisms, cell lines, whole tissue, blood, serum, and plasma using the spectrophotometer or plate reader. Available in 500mL or 1000mL assay quantities.
We have two kits for total protein assay. The ITSIPrep Bradford ToPA kit contains all the reagents, protein standards in a ready-to-use format, cuvettes (K-0014-20) and microtiter plate (K-0014-96) for easy and accurate measurement of total protein concentration. ToPA is based on the Bradford assay and specifically developed to eliminate the common errors associated with total protein quantitation. K-0014-20 (20 Assays), K-0014-96 (96 Assays).
The easy-to-use Protein Precipitation Kit (ToPREP) is specifically formulated for precipitation of proteins from solution with higher than 90% recovery. It contains the ProPreCip protein precipitation booster, optimized reagents, and standard operating procedure. The precipitated protein is suitable for 1D-PAGE, 2D-PAGE, 2D-DIGE, western analysis and mass spectrometry.