ITSIPrep Protein Digestion Monitoring (ProDM) Kit is the first kit to allow fast and accurate monitoring of the protein digestion step without performing gel electrophoresis. ProDM kit will allow researchers easily and quickly test the trypsinized sample to accurately determine the extent of digestion. All enzymes can be monitored including trypsin, chymotrypsin.
ITSI-BIO Buccal DNA Collection Kit (K-0022) is an easy-to-use kit validated specifically for the collection of buccal DNA sample. It is a reliable and inexpensive alternative to the more expensive kits. It can be used to collect cheek cells from which DNA will be isolated.
ITSI-Biosciences distinctive Western Blotting Kit (W-Blot™ [K-0045]) is a ready-to-probe western blot of human, animal, or plant proteins on a nitrocellulose membrane (Figure 1, Figure 2). Each blot contains 20ug of total protein per lane. W-Blot™ can be re-probed multiple times and can be customized with any required tissue. The PVDF membrane is available upon request.
ITSIPrep Trypsin Digestion Monitoring (TrypDM) Kit is the first kit to allow fast and accurate monitoring of the level of protein digested when trypsin or any other enzyme is used. No gel electrophoresis is required to confirm that the protein is digested. The TrypDM kit will enable researchers to easily and quickly test the trypsinized sample to accurately determine the extent of digestion.
This Proteomics Grade Plasma Tube (PGPT) is specifically developed for collection of plasma specimens. PGPT contains Gel, Lithium Heparin and five-protease inhibitors with specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. PGPT reagents instantly inactivate proteases thereby preventing changes in the proteome attributable to ex-vivo protease activity.
This Proteomics Grade Serum Tube (PGST) is developed specifically for collection of serum specimens. The tube contains five-protease inhibitors with specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. All samples can be analyzed by mass spectrometry, SDS-PAGE, 2D-DIGE and Western blotting.
The Quanti-Protein Assay Reagent – Nano (nQ-PAR) is an optimized ready-to-use reagent for quantitation of total proteins using the NanoDrop ND1000 spectrophotometer. nQ-PAR can be used to quantify proteins isolated from microorganisms, cell lines, whole tissue, blood, serum and plasma. nQ-PAR is compatible with many procedures, can be stored at room temperature, and tolerates many common laboratory buffers. Available in 50 or 100 assays.
The Protein Standard Curve Reagent kit provides eight concentrations (25ug/mL – 2000ug/mL) of Bovine Serum Albumin in a ready-to-use and room temperature stable format. This product improves reproducibility and reduces the time required for Bradford assay-based standard curve preparation, and hence accuracy and ease of protein quantitation. Available in 50 or 100 assays.
The Quanti-Protein Assay Reagent (Q-PAR) is an optimized ready-to-use reagent for quantitation of total protein. Q-PAR can be used to quantify proteins isolated from microorganisms, cell lines, whole tissue, blood, serum, and plasma using the spectrophotometer or plate reader. Available in 500mL or 1000mL assay quantities.
We have two kits for total protein assay. The ITSIPrep Bradford ToPA kit contains all the reagents, protein standards in a ready-to-use format, cuvettes (K-0014-20) and microtiter plate (K-0014-96) for easy and accurate measurement of total protein concentration. ToPA is based on the Bradford assay and specifically developed to eliminate the common errors associated with total protein quantitation. K-0014-20 (20 Assays), K-0014-96 (96 Assays).
The easy-to-use Protein Precipitation Kit (ToPREP) is specifically formulated for precipitation of proteins from solution with higher than 90% recovery. It contains the ProPreCip protein precipitation booster, optimized reagents, and standard operating procedure. The precipitated protein is suitable for 1D-PAGE, 2D-PAGE, 2D-DIGE, western analysis and mass spectrometry.
ToPI-Blood (K-0023) is a validated kit and procedure for isolation of proteins from blood cells. The resulting isolated proteins can be analyzed by mass spectrometry, western blotting, 2D-DIGE, SDS-PAGE and other downstream applications.
ToPI –P includes a protease inhibitor cocktail that inhibits proteases present in plants and protect the proteins from proteolytic degradation during the isolation procedure. The isolated protein can be analyzed by electrophoresis (1D, 2D and 2D-DIGE) and mass spectrometry. ToPI-P contains all the buffers necessary for the successful isolation of proteins from plants, fruits, leaves, seeds and roots.
The ToPI-U mini kit contains optimized and ready-to-use reagents and concentration devices for processing of up to 20 urine samples. More than 200 ug of proteins can be isolated from 1 mL of urine. The ITSIPREP ToPI-U is a validated kit specifically designed for isolating and concentrating proteins from urine. The concentrated proteins are compatible with variety of downstream proteomics analysis including electrophoresis (1D, 2D and 2D-DIGE) and western analysis.
The ToPI-F2 is developed specifically to permit the isolation of total proteins from Formalin Fixed Paraffin Embedded (FFPE) specimen for 1D-PAGE and mass spectrometry. ToPI-F2 has been used to isolate proteins from FFPE samples stored for up to 5-10 years. The protocol and reagents provided in the kit is sufficient for isolating protein from 10 x 5 (10u thick) specimens. Approximately 5,000 ug/ml – 10,000 ug/ml of total protein can be extracted from 5 sections (~10u thick each) obtained from samples fixed with 10% buffered formalin and stored for up to 5 years.
ToPI-PAGE is optimized for isolation of total proteins from cell lines (K-0011-C) and mammalian tissue (K-0011-T) prior to sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). The standard ToPI kit will allow quick and convenient isolation of proteins from 10 samples.
ToPI-DIGE is optimized for isolation of total proteins from cell lines (K-0010-C) and mammalian tissue (K-0010-T) for 2-Dimensional difference in gel-electrophoresis (2D-DIGE). Using the kit prevents protein breakdown and prevents additional buffer exchange steps that may lead to protein breakdown and lower yield.
BioBlue™ preferentially binds to proteins forming dark blue and distinct protein bands. BioBlue™ is sensitive and reveals many more low-abundant protein bands compared to competing Coomassie R-250 and G-250 based stains because of the decreased staining of the gel. The stained proteins can be easily visualized and picked/cut from the gel without the methanol or acetic acid destaining step, making the product easy/fast-to-use and environmentally friendly. BioBlue™ is fully compatible with mass spectrometry and western analysis.
The ITSIPrep ProFEK (K-0015) Protein Fraction and Enrichment Kit (ProFEK) provides a validated, fast, and cost-effective method to fractionate and enrich for membrane, cytosol, and nuclear proteins without ultra centrifugation. The optimized reagents and procedure provided with the kit allow for reproducible results and identification of unique region associated proteins. The extracted membrane, nuclear, and/or cytoplasmic protein fractions can be analyzed by mass spectrometry, SDS-PAGE, 2D-PAGE, 2D-DIGE and western blotting. Includes a Disposable Pestle/Tube for sample homogenization.
Mixture of protease inhibitors for EDTA-free sample preparation. PIC-4 contains Aprotinin, AEBSF, E-64 and Leupeptin. The cocktail is optimized to inhibit the activity of serine and cysteine proteases and will produce 1mL of a 100X solution.
Mixture of protease inhibitors for preparation of bacterial cell extracts. PIC-3 contains EDTA, AEBSF, Bestatin, E-64 and Pepstatin A. The cocktail inhibits a wide variety of proteases, including; aspartic, cysteine, serine, metalloproteases, and aminopeptidases. The preparation is sufficient for 20g of cells.
Mixture of protease inhibitors for preparation of mammalian cells and tissue prior to analysis. It will inhibit many proteases including; aspartic, cysteine, serine, metalloproteases, and aminopeptidases. The cocktail is provided as a DMSO suspension suitable for processing up to 20g of cells or tissue.
Mixture of protease inhibitors for sample preparation. I-PIC.1 contains aprotinin, AEBSF and EDTA. It will inhibit many proteases including; aspartic, cysteine, serine, metalloproteases, and aminopeptidases. The cocktail will produce 1mL of a 100X solution and inhibits the activity of a wide variety of proteases.
The ITSIPrep lysates are useful for a wide range of applications, including use as standards or controls for SDS-PAGE, 2D-DIGE, mass spectrometry and Western blot analysis. All ITSIPrep lysates are supplied in 100ug. If the tissue or species you require is not available let us know, and we can obtain the tissue you need. The following human protein lysates are in stock, Lung, Heart, Spleen, Adrenal Gland, Trachea, Pancreas, Thyroid, Breast, Myometrium and Duodenum.
Proteomics grade Tris (10x) Buffered Saline is used in biochemical and molecular biology applications. The 10x solution contains 0.25 M Tris/Tris-HCl, 1.37M Sodium Chloride, and 0.027M Potassium Chloride. Each kit contains 500ml or 1000ml of buffer.
SDS-PAGE (2X) Loading Buffer is added to protein samples prior to electrophoresis on SDS-PAGE gels. The loading buffer contains 2% SDS, 62.5mM Tris-HCl (pH 6.8), 25% Gylcerol, and 0.01% Bromophenol Blue. Each kit contains 30ml of buffer.
Proteomics grade Phosphate (10X) Buffered Saline is used in biochemical and molecular biology applications. The 10x solution contains Sodium Chloride, Potassium Phosphate – monobasic, Sodium Phosphate – dibasic and Potassium Chloride. Available in 500mL and 1000mL.
The Native Protein Extraction from Cells and Tissues Buffer (NPB) is for isolation of proteins in the native state from cells and tissue. This buffer can be used to prepare samples for ELISA, immunoprecipitation or other immunoassays where denaturing of proteins or the presence of strong detergents may interfere with the assay. NPB is provided in 2X concentrated Tris buffered saline, with a pH of 7.4 when diluted to the 1X working concentration.
Proteomics grade Equilibration Buffer for 2D-DIGE and 2D-PAGE. Each kit contains 200ml of equilibration buffer made up of Urea, 50% Glycerol, 35% Sodium Dodecyl Sulphate, and 2.5% Tris (hydroxymethyl) aminomethane.
ITSI-Biosciences’ unique Autoantibody Profiling System (iAPS) is specifically developed for rapid identification, discovery and quantitation of multiple autoantibodies that may be present in clinical and experimental specimens. The profiling system consists of a 96-well microtiter plate and the kit includes all reagents and materials required for screening, detection and interpretation of the results.
ITSIPrep ASKs (K-0013) is a validated and cost effective organic solvent-based protocol for selective depletion of albumin (Alb) from serum and plasma samples and analysis of the albumin-enriched fraction (Alb+) and albumin depleted (Alb-) fractions. Treated samples can be analyzed by electrophoresis (1D-PAGE, 2D-PAGE, 2D-DIGE), mass spectrometry and western analysis.
ITSIPrep ASKc (K-0012) is a validated spin column based protocol for depletion of albumin (Alb) from serum and plasma samples prior to analysis of the albumin enriched fraction (Alb+) and/or albumin depleted (Alb-) fraction. Treated samples can be analyzed by electrophoresis, mass spectrometry and western analysis.