Human Serum Albumin (HSA, 66 kDa) typically accounts for 65% or more of the total protein present in serum and plasma samples. This can prevent the detection of low abundant plasma and serum proteins in most experiments. Albumin also distorts lanes during electrophoresis if a large amount of total protein is loaded to permit the detection of low abundant proteins. Albumin can also mask other proteins that migrate around 50-70 kDa thereby preventing their detection. Benefits of using ITSIPrep Albumin Removal Kits:

  • Lowest cost/sample
  • Ready-to-use reagents
  • Reproducible results
  • Depletes Greater than 80% albumin
  • Easy-to-follow protocol

ITSIPrep ASKc (K-0012) is a validated spin column based protocol for depletion of albumin (Alb) from serum and plasma samples prior to analysis of the albumin enriched fraction (Alb+) and/or albumin depleted (Alb-) fraction. More than 90% of albumin is depleted from whole serum using the ITSIPrep ASKc Kit (see Figure). This protocol has been widely applied in many laboratories for albumin removal to reveal lesser abundant proteins in the Alb- fraction or albumin-associated proteins in the Alb+ fraction. Treated samples can be analyzed by electrophoresis, mass spectrometry and western analysis.

Each Kit contains optimized buffer systems and consumables including:

  • Albumin Segregation Matrix (ASM
  • ASM Buffer 1
  • ASM Buffer 2
  • ASM Buffer 3
  • Microspin Columns containing ASM
  • 5KDa MWCO centrifugation Device
  • 2.0 ml Centrifuge tubes