ITSI – Biosciences also offers SDS PAGE, large format Two Dimensional – Difference in Gel Electrophoresis (2D-DIGE) and 1D/2D Western Blotting services. SDS PAGE can be performed with a mini gel or the Agilent BioAnalyzer and biochip. In each case, an automated software program is used to detect, quantify and annotate the protein bands.

We can also screen for phosphoproteins, by staining the 2D-PAGE gels with a phosphor protein stain. The 1D and 2D Gels can also be blotted by western transfer and screened with antibodies supplied by the client.

 

We offer the following services:

S/NCAT NO.NAMEDESCRIPTIONUNIT COST
1SP1Sample Preparation 1Homogenization, protein quantitation using ToPA Bradford protein assay kit.Per sample
2SP2Sample Preparation 2Includes, precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA kits.Per sample
3SP3Sample Preparation 3Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Column kit.Per sample
4SP4Sample Preparation 4Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Solvent kit.Per sample
5SP5Sample Preparation 5Depletion of 14 abundant proteins from serum / plasma using IgY column.Per sample
6SP9Sample Preparation 9Urine protein isolation and concentration. Service includes Isolation, partial purification, and concentration of urine proteins using ITSI Urine Protein Isolation kit.Per sample
7S10MG1D SDS-PAGE – Mini Gel1D gel electrophoresis. The service includes

  • Determining Protein Concentration
  • SDS-PAGE,
  • Gel scanning and
  • Image analysis.
1-8 samples
8S10BA1D SDS-PAGE – Bioanalyzer1D Gel electrophoresis using Agilent Bioanalyzer and Chip. The service includes

  • Determining protein concentration
  • SDS-PAGE with the bioanalyzer, and
  • Image analysis.
1-8 samples
9S202D-PAGEClassical Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE). The service includes

  • Determining Protein Concentration
  • 1st dimension,
  • 2nd dimension,
  • Staining,
  • De-staining and
  • Scanning.
1 sample Per Gel
10S20P2D-PAGE PhosPhoPhospho protein profiling. The service includes

  • Determining Protein Concentration
  • 1st dimension,
  • 2nd dimension,
  • Staining,
  • De-staining,
  • Phosphoprotein staining and
  • Scanning.
Per gel
11S101-1D1D-Western Blotting1D Western analysis using client supplied antibodyPer blot
12S101-2D2D-Western Blotting2D Western analysis using client supplied antibodyPer blot
13SP7Sample preparation 7Manual In-gel digestion. Digestion of proteins in a gel band/spot using trypsin. The service includes

  • Gel de-staining – coomassie or silver stain
  • Alkylation and reduction
  • Trypsin digestion
  • Peptide extraction from the gels for mass spectrometry analysis.
Per sample
14SP10Sample preparation 10Zip tip cleanup of in-gel or in-solution digested samples….Per sample
15S50.60LC60-MS/MSPeptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of pure protein samples. Services include

  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search and
  • Identified protein annotation – 60 min run.
Per sample
16DBS-PIDDatabase Search – Protein IdentificationDatabase search for protein identification. The service includes

  • Downloading the most recent database files from NCBI or other relevant sources
  • Database search and
  • Identified protein annotation.
Per sample
17DBS-PTMDatabase Search – Protein Identification and PTM MappingDatabase search for protein identification and post translational modification mapping. The service includes

  • Downloading the most recent database files from NCBI or other relevant sources
  • Database search and
  • Identified protein annotation.
Per sample
18GOGene Ontology (GO) AnnotationAnnotation of proteins using GO classification for biological process, molecular function and cellular component.Per sample

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