Total Protein Assay Kit (ToPA, Cat #: K-0014)

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Protein concentration measurement is usually the first step in most protein analysis work-flow processes. The criteria for choice of a protein assay method are usually based on convenience, availability of adequate amount of protein for assay, presence or absence of interfering agents in the mixture, and need for accuracy. For example, the Lowry protein assay… Read more »

Protein Isolation Kit for BLOOD [ToPI-B; Cat #: K-0023]

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Recently, there has been increased interest in the analysis of proteins from blood, partly because of the potential of identifying candidate biomarkers via a minimally invasive procedure. The proteins excreted in blood can be used as biomarkers in the diagnosis of kidney, urinary tract, coronary artery disease and other systemic diseases. The isolation of blood… Read more »

Protein Isolation Kit for PLANT TISSUE [ToPI-P; Cat #: K-0025]

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ToPI-P is used specifically to isolate proteins from plant tissue. ToPI –P includes a protease inhibitor cocktail that inhibits proteases present in plants and protect the proteins from proteolytic degradation during the isolation procedure. ToPI-P contains all the buffers necessary for the successful isolation of proteins from plants, fruits, leaves, seeds and roots. Fig 1:… Read more »

Protein Isolation Kit for URINE SAMPLES [ToPI-U; Cat #: K-0019]

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Recently, there has been increased interest in the analysis of urine proteins, partly because of the potential of identifying candidate biomarkers via a minimally invasive procedure. The proteins excreted in urine can be used as biomarkers in the diagnosis of kidney, urinary tract, coronary artery disease and other systemic diseases. The isolation of urine proteins… Read more »

Protein Isolation Kit for Formalin-Fixed Paraffin Embedded Samples [ToPI-F2; Cat #: K-0017]

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Formalin-fixed paraffin-embedded (FFPE) specimens have been largely ignored because of the recognized difficulty in isolation of proteins from such specimens. The analysis of formalin-fixed paraffin-embedded (FFPE) specimens is desirable because FFPE are the largest and longest time-spanning collections of patient materials in pathology archives around the world. Moreover, because the clinical records are available, and… Read more »

Total Protein Isolation Kit [ToPI; Cat #: K-0011]

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The ToPI-PAGE kit contains a set of optimized buffers, consumables, and standard operating procedure that allow the isolation of total proteins from biological and clinical samples with no protein breakdown. ToPI-PAGE is optimized for isolation of total proteins from cell lines (K-0011-C) and mammalian tissue (K-0011-T) prior to sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE)…. Read more »

Protein Isolation Kit for Two-Dimensional Difference Gel Electrophoresis [ToPI-DIGE; Cat #: K-0010]

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The ToPI-DIGE™ Kit contains a set of optimized reagents and standard operating procedures that allow basic and clinical research scientists to easily, conveniently and reproducibly isolate total proteins from human and non-human cell lines (attached and suspended), solid tissue and tumor biopsies for use in 2D-DIGE experiments (Fig 1). ToPI-DIGE is optimized for isolation of… Read more »